Autoregulation of fos: the dyad symmetry element as the major target of repression. |
| |
| Authors: | H K?nig H Ponta U Rahmsdorf M Büscher A Sch?nthal H J Rahmsdorf P Herrlich |
| |
| Affiliation: | Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, FRG. |
| |
| Abstract: | Fos and Jun co-operatively repress the fos promoter. Removal of all putative Fos/Jun binding sites from the fos promoter neither obliterates the repression by Fos/Jun in transient cotransfection experiments in NIH3T3 cells nor the turn-off kinetics of serum-induced fos expression in stably transfected NIH3T3 cells. The dyad symmetry element (DSE) suffices to subject a promoter to this type of repression. However, one of the putative Fos/Jun binding sites (-292 to -299 and thus located immediately adjacent to the DSE), determines the very low level of basal expression. |
| |
| Keywords: | |
|
|
