Expression of nodule-specific uricase in soybean callus tissue is regulated by oxygen |
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| Authors: | Larsen Knud Jochimsen Bjarne U |
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| Affiliation: | Department of Molecular Biology and Plant Physiology, University of Aarhus, DK-8000 Aarhus C, Denmark. |
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| Abstract: | In soybean root nodules the enzyme uricase is expressed concomitantly with nodule development. The initial expression of this protein does not depend on active nitrogen fixation, as demonstrated by analysis of uricase activity in effective and ineffective root nodules. However, the maximal level of uricase activity is determined by the infecting Rhizobium japonicum strain. Sterile root cultures and callus tissue, devoid of the microsymbiont, were incubated at varying oxygen concentrations and analyzed for uricase activity. The specific activity of uricase was increased by lowering the oxygen concentration, with the highest activity obtained around 4−5% oxygen. The increase in uricase activity was due to increased uricase synthesis, as demonstrated by in vivo labelling of callus culture followed by immunoprecipitation with antibodies raised against highly purified nodule uricase. |
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