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蚕豆蛋白质亚基分析与特异种质鉴定
引用本文:刘玉皎,侯万伟,石建斌.蚕豆蛋白质亚基分析与特异种质鉴定[J].西北植物学报,2012,32(1):54-59.
作者姓名:刘玉皎  侯万伟  石建斌
作者单位:1. 青海省农林科学院,西宁810016;青海省高原作物种质资源创新与利用-国家重点实验室培育基地,西宁810016
2. 青海大学,西宁,810016
基金项目:国家973计划前期专项,现代农业产业技术体系建设专项,青海高校"135高层次人才培养工程"
摘    要:采用SDS-PAGE方法,对112份不同基因型蚕豆的清蛋白和球蛋白亚基的差异性进行分析。结果显示:(1)蚕豆清蛋白和球蛋白亚基的有效等位变异分别为1.750 0±0.452 3、1.545 5±0.522 2,多态性比率分别为75.00%、54.55%,清蛋白的亚基遗传多样性指数较球蛋白亚基高。(2)蚕豆清蛋白和球蛋白分别含有在不同基因型蚕豆中构成不同的12和10个亚基,其中清蛋白含有9个基本亚基,116kD、96kD、45kD为清蛋白的3个特异亚基;球蛋白含有8个基本亚基,58kD、35kD为球蛋白的2个特异亚基;研究共鉴定筛选出42个含有清蛋白特异亚基和21个含有球蛋白亚基的种质资源。(3)清蛋白的97kD、63kD基本亚基和球蛋白的97kD、56kD、47kD基本亚基存在一定的缺失现象,共鉴定出19个清蛋白亚基和21个球蛋白亚基缺失的优异种质。研究表明,蚕豆清蛋白和球蛋白亚基构成在不同种质之间具有差异性,除基本亚基外,部分种质还含有特异亚基或缺失亚基。

关 键 词:蚕豆  蛋白亚基  多样性  种质鉴定

Identification of Special Gene and Analysis of the Protein-Subunit for different Genotypes of Faba Bean
LIU Yu-jiao,HOU Wan-wei,SHI Jian-bin.Identification of Special Gene and Analysis of the Protein-Subunit for different Genotypes of Faba Bean[J].Acta Botanica Boreali-Occidentalia Sinica,2012,32(1):54-59.
Authors:LIU Yu-jiao  HOU Wan-wei  SHI Jian-bin
Affiliation:1 Qinghai Academy of Agriculture and Forestry Science,Xining 810016,China;2 The Cultivating Base of National Key Lab-Plateau Crop Gene Innovated and Utilized in Qinghai Province,Xining 810016,China;3 Qinghai University,Xining 810016,China)
Abstract:The differentce of protein subunits of 112 faba bean was analyzed by SDS-PAGE,in order to find out specific protein subunits.The result showed that:(1)the effective number of albumin and globulin alleles was 1.750 0±0.452 3,1.545 5±0.522 2,and the percentages of polymorphic subunit was 75.00%,54.55%,respectively.(2)It was difference between the albumin and globulin subunit numbers in different genotypes,containing 12 albumin subunits and 10 globulin subunits thereof the specific albumin subunits were 116 kD,96 kD,45 kD and specific globulin subunits are 58 kD,35 kD.42 varieties contain specific albumin subunit,21 varieties contain specific globulin subunit.(3)The lack subunits were 97 kD,63 kD of albumin and 97 kD,56 kD,47 kD of globulin.19 varieties were found with based albumin subunit lack and 21 varieties with globulin subunit lack.In summary,both albumin and globulin subunit numbers are different among different faba bean genotypes.Besides the based subunits,some have specific subunits and some have lack subunit.
Keywords:faba bean  diversity  protein subunit  gene identification
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