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H_2S对干旱胁迫下白三叶幼苗抗氧化防御能力的影响
引用本文:何小双,蒋晓琪,彭丹丹,彭 燕.H_2S对干旱胁迫下白三叶幼苗抗氧化防御能力的影响[J].西北植物学报,2017,37(1):88-96.
作者姓名:何小双  蒋晓琪  彭丹丹  彭 燕
作者单位:(草业科学重点实验室 四川农业大学 动物科技学院,成都611130)
基金项目:国家自然科学基金项目(31372371);
摘    要:以‘拉丁诺’白三叶(Trifolium repens cv.‘Ladino’)为试验材料,研究外源H2S处理对PEG6 000(聚乙二醇)模拟干旱胁迫下白三叶叶片相对含水量(RWC)、膜脂过氧化、活性氧成分、抗氧化酶、抗坏血酸-谷胱甘肽循环代谢和非酶抗氧化物质的影响,以揭示H_2S调控白三叶抗旱性的生理机制。结果显示:(1)0.2 mmol/L的外源NaHS(H_2S供体)能显著提高干旱胁迫下白三叶的叶片相对含水量,维持显著较低的电解质渗透率(EL)和丙二醛(MDA)含量。(2)与直接干旱胁迫相比,干旱胁迫下外源添加NaHS处理的白三叶叶片内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性显著增强,抗坏血酸-谷胱甘肽循环代谢中关键酶抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、单脱水抗坏血酸还原酶(MDHAR)和谷胱甘肽还原酶(GR)活性及其抗氧化中间产物抗坏血酸(AsA)、谷胱甘肽(GSH)含量也显著提高。(3)叶片类黄酮、总酚和原花青素的含量在一定的胁迫时间范围内亦显著增加,并伴随着活性氧成分O_2~(-·)产生速率和H_2O_2水平降低。研究认为,外源H2S能通过促进干旱胁迫下白三叶体内的多重抗氧化防御能力来提高其幼苗的抗旱性。

关 键 词:白三叶  干旱胁迫  H2S  抗氧化保护

Effects of Hydrogen Sulfide on Antioxidant Defense of White Clover Leaves in Response to Drought Stress
HE Xiaoshuang,JIANG Xiaoqi,PENG Dandan,PENG Yan.Effects of Hydrogen Sulfide on Antioxidant Defense of White Clover Leaves in Response to Drought Stress[J].Acta Botanica Boreali-Occidentalia Sinica,2017,37(1):88-96.
Authors:HE Xiaoshuang  JIANG Xiaoqi  PENG Dandan  PENG Yan
Abstract:The effects of exogenous hydrogen sulfide (H2S) on relative water content, membrane lipid peroxidation, reactive oxygen species, antioxidant enzyme activities, AsA GSH cycle metabolism, and antioxidants were investigated with white clover (Trifolium repens) under drought stress induced by PEG 6000, which reveals the physiological mechanism of H2S regulated drought tolerance in white clover. The results showed that: (1) the plants treated with 0.2 mmol/L sodium hydrosulfide (NaHS), a H2S donor, maintained higher leaf relative water content and lower electrolyte leakage and MDA content under drought stress as compared to the plants without the pretreatment with NaHS. (2) Similarly, the exogenous NaHS effectively improved SOD, CAT, POD activities and accelerated AsA GSH cycle metabolism via the improvement of the key enzyme activities (APX, DHAR, MDHAR and GR), AsA and GSH content. (3) The application of NaHS also increased flavonoid, total phenols and proanthocyanidins contents in leaves of white clover in a period of time, followed by decreases in the generation rate of O-·2 and H2O2 content in response to drought stress. Therefore, this study suggests that exogenous H2S can improve drought tolerance of white clover seedlings through enhancing the leaf antioxidant defense system.
Keywords:white clover  drought stress  H2S  antioxidant defense
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