Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency,proliferation, and differentiation |
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| Authors: | Song Park Yonghun Sung Jain Jeong Minjee Choi Jinhee Lee Wookbong Kwon Soyoung Jang Si Jun Park Jae Young Kim Sung Hyun Kim Duhak Yoon Zae Young Ryoo Myoung Ok Kim |
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| Affiliation: | 1. School of Life Science, BK21 plus KNU Creative Bio Research Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea;2. Core Protein Resources Center, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea;3. Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea;4. China‐US (Henan) Hormel Cancer Institute, Zhengzhou, Henan 450008, China;5. The School of Animal BT Science, Kyungpook National University, Sangju‐si, Gyeongsangbuk‐do, South Korea |
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| Abstract: | Induced pluripotent stem (iPS) cells are important for clinical application and stem cell research. Although human melanoma‐associated antigen A2 (hMAGEA2) expression is known to affect differentiation in embryonic stem cells, its specific role in iPS cells remains unclear. To evaluate the function of hMAGEA2 and its characteristics in iPS cells, we produced hMAGEA2‐overexpressing iPS cells from hMAGEA2‐overexpressing transgenic mice. Although the iPS cells with overexpressed hMAGEA2 did not differ in morphology, their pluripotency, and self‐renewal related genes (Nanog, Oct3/4, Sox2, and Stat3), expression level was significantly upregulated. Moreover, hMAGEA2 contributed to the promotion of cell cycle progression, thereby accelerating cell proliferation. Through embryoid body formation in vitro and teratoma formation in vivo, we demonstrated that hMAGEA2 critically decreases the differentiation ability of iPS cells. These data indicate that hMAGEA2 intensifies the self‐renewal, pluripotency, and degree of proliferation of iPS cells, while significantly repressing their differentiation efficiency. Therefore, our findings prove that hMAGEA2 plays key roles in iPS cells. |
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| Keywords: | differentiation hMAGEA2 induced pluripotent stem cells pluripotency proliferation |
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