Study of differentiated human umbilical cord–derived mesenchymal stem cells transplantation on rat model of advanced parkinsonism |
| |
| Authors: | Zhaowei Wang Aimin Chen Shengjuan Yan Chengyan Li |
| |
| Affiliation: | 1. Dept of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P. R .C;2. Qianjiang Central Hospital, Qianjiang 433100, Hubei Province, P. R .C |
| |
| Abstract: | The aim of this study was to explore the curative effect of differentiated human umbilical cord–derived mesenchymal stem cells (hUC‐MSCs) transplantation on rat of advanced Parkinson disease (PD) model. Human umbilical cord–derived mesenchymal stem cells were cultured and induced differentiation in vitro. The PD rats were established and allocated randomly into 2 groups: differentiated hUC‐MSCs groups and physiological saline groups (the control group). Rotation test and immunofluorescence double staining were done. The result showed that hUC‐MSCs could differentiate into mature dopamine neurons. Frequency of rotation was significantly less in differentiated hUC‐MSCs groups than in normal saline group. After we transplanted these cells into the unilateral lesioned substantia nigra induced by striatal injection of 6‐hydroxydopamine and performed in the medial forebrain bundle and ventral tegmental area, nigral tyrosine hydroxylase–positive cells were observed and survival of at least 2 months. In addition, transplantation of hUC‐MSCs could make an obviously therapeutic effect on PD rats. |
| |
| Keywords: | human umbilical cord– derived mesenchymal stem cells Parkinson disease transplantation tyrosine hydroxylase |
|
|
