Analysis and comparison of nucleotide sequences encoding the genes for [NiFe] and [NiFeSe] hydrogenases from Desulfovibrio gigas and Desulfovibrio baculatus. |
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| Authors: | G Voordouw N K Menon J LeGall E S Choi H D Peck Jr and A E Przybyla |
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| Affiliation: | Department of Biological Sciences, University of Calgary, Alberta, Canada. |
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| Abstract: | The nucleotide sequences encoding the NiFe] hydrogenase from Desulfovibrio gigas and the NiFeSe] hydrogenase from Desulfovibrio baculatus (N.K. Menon, H.D. Peck, Jr., J. LeGall, and A.E. Przybyla, J. Bacteriol. 169:5401-5407, 1987; C. Li, H.D. Peck, Jr., J. LeGall, and A.E. Przybyla, DNA 6:539-551, 1987) were analyzed by the codon usage method of Staden and McLachlan. The reported reading frames were found to contain regions of low codon probability which are matched by more probable sequences in other frames. Renewed nucleotide sequencing showed the probable frames to be correct. The corrected sequences of the two small and large subunits share a significant degree of sequence homology. The small subunit, which contains 10 conserved cysteine residues, is likely to coordinate at least 2 iron-sulfur clusters, while the finding of a selenocysteine codon (TGA) near the 3' end of the NiFeSe] large-subunit gene matched by a regular cysteine codon (TGC) in the NiFe] large-subunit gene indicates the presence of some of the ligands to the active-site nickel in the large subunit. |
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