Cloning of the gene coding for Staphylococcus hyicus exfoliative toxin B and its expression in Escherichia coli |
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Authors: | Watanabe T Sato H Hatakeyama Y Matsuzawa T Kawai M Aizawa C Danbara H Maehara N |
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Affiliation: | Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. |
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Abstract: | The Staphylococcus hyicus exfoliative toxin B (SHETB) gene was cloned into pUC118 and expressed in Escherichia coli. The nucleotide sequence of the SHETB gene consists of a coding region of 804 bp specifying a polypeptide of 268 amino acid residues, which included a putative 20-residue signal sequence. |
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