| 抗细胞趋化因子CCL21单克隆抗体处理对小鼠急性心肌梗死后左心室重构及心功能的影响 |
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| 引用本文： | 蒋易,燕艳丽,白建文.抗细胞趋化因子CCL21单克隆抗体处理对小鼠急性心肌梗死后左心室重构及心功能的影响[J].中国应用生理学杂志,2018,34(3):197-200. |
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| 作者姓名： | 蒋易 燕艳丽 白建文 |
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| 作者单位： | 同济大学附属东方医院急诊内科, 上海 200120 |
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| 基金项目： | 白建文,Tel:021-38804518;E-mail:bjwhos@163.com |
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| 摘 要： | 目的：探讨抗CCL21单克隆抗体处理对小鼠急性心肌梗死后心室重构和心功能的影响。方法：C57BL/6小鼠随机分为假手术组、模型组和CCL21单抗干预组，并进一步分为1、3、7和21 d亚组。采用结扎冠状动脉左前降支的方法构建小鼠急性心肌梗死模型，在冠状动脉结扎后5 min和第3天，模型组小鼠静脉注射isotype-IgG 1.0 mg，CCL21单抗干预组小鼠静脉注射山羊抗小鼠CCL21单克隆抗体1.0 mg。建模后，Western blot法检测急性心肌梗死后第1、3、7天心肌组织CCR7表达，检测急性心肌梗死后第7天心肌组织MMP-2和MMP-9表达；建模后第1、3、7天，ELISA法检测各组小鼠血清TNF-α和IL-6水平，每组检测8只小鼠。在建模后第7天和21天，超声心动图法评估左心室功能变化。结果：与假手术组比较，模型组小鼠急性心肌梗死后血清CCL21、TNF-α和IL-6及心肌组织CCR7、MMP-2、MMP-9明显升高（P<0.05）；与模型组比较，CCL21单抗干预组小鼠血清TNF-α和IL-6及梗死区心肌组织MMP-9水平明显降低（P<0.05）。结论：抗CCL21单克隆抗体处理，通过抑制梗死后炎症反应及MMP-9表达水平发挥防止小鼠心脏重构和保护左心室功能的效应。
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| 关 键 词： | 心肌梗死 炎症反应 MMP-9 心脏重构 CCL21 小鼠 |
| 收稿时间： | 2017-03-07 |
Effect of treating with anti-CCL21 monoclonal antibody on left ventricular remodeling and cardiac function post-acute myocardial infarction in mice |
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| JIANG Yi,YAN Yan-li,BAI Jian-wen.Effect of treating with anti-CCL21 monoclonal antibody on left ventricular remodeling and cardiac function post-acute myocardial infarction in mice[J].Chinese Journal of Applied Physiology,2018,34(3):197-200. |
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| Authors: | JIANG Yi YAN Yan-li BAI Jian-wen |
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| Affiliation: | Department of Internal Medicine, Emergency Center, East Hospital, Tongji University School of Medicine, Shanghai 200120, China |
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| Abstract: | Objective: To explore the therapeutic effect of treating with anti-CCL21 monoclonal antibody on left ventricular remodeling and cardiac function post-acute myocardial infarction in mice.Methods: C57BL/6 mice were randomly divided into sham-operated group, model group and anti-CCL21 monoclonal antibody intervention group, and further randomly divided into 1 d, 3 d,7 d and 21 d subgroups. A mouse model of acute myocardial infarction (AMI) was generated by left anterior descending coronary artery ligation in C57BL/6 mice. Mice in model group were intravenously given isotype-IgG 1.0 mg, mice in anti-CCL21 monoclonal antibody intervention group were intravenously given goat anti-mouse CCL21 monoclonal antibody 1.0 mg at 5 minute and on the 3rd day post-coronary artery ligation. After surgery, C-C motif chemokine receptor 7 (CCR7) expression in myocardium was detected on the 1st, 3rd, 7th day, and the expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 in myocardium were detected on the 7th day 8 mice per group using Western blot. Serum levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in each group were measured on the 1st, 3rd, 7th day post-surgery using ELISA, 8 mice per group. Transthoracic echocardiography was underwent in mice on the 7th and 21th day after surgery using an echocardiography system to evaluate left ventricular function.Results: Compared with sham-operated group, serum levels of CCL21, TNF-α, IL-6, and myocardiac expression of CCR7、MMP-2、MMP-9 in model group were significantly increased (P<0.05). Compared with model group, serum levels of TNF-α and IL-6 and myocardiac expression level of MMP-9 were significantly decreased in anti-CCL21 monoclonal antibody intervention group(P<0.05).Conclusion: Our study indicates that treating with anti-CCL21 monoclonal antibody is involved in preventing cardiac remodeling and protecting left ventricular function post AMI via inhibiting inflammatory response and MMP-9 expression level. |
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| Keywords: | myocardial infarction inflammatory response MMP-9 cardiac remodeling CCL21 mice |
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