| 花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制 |
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| 引用本文： | 王静,陈泽峰,和春霞,王莉莉,郑嘉慧,张皓,王占磊,鲁亚平.花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制[J].中国应用生理学杂志,2018,34(5):476-480. |
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| 作者姓名： | 王静 陈泽峰 和春霞 王莉莉 郑嘉慧 张皓 王占磊 鲁亚平 |
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| 作者单位： | 1. 皖南医学院基础医学院生理学教研室, 安徽 芜湖 241002;
2. 皖南医学院2013级临床专业, 安徽 芜湖 241002;
3. 皖南医学院2015级临床专业, 安徽 芜湖 241002;
4. 皖南医学院2014级麻醉专业, 安徽 芜湖 241002;
5. 安徽师范大学生命科学学院, 安徽 芜湖 241002 |
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| 基金项目： | 国家自然科学基金面上项目（81671586）；安徽省教育厅高校优秀人才计划项目（gxyq2017036）；皖南医学院重点科研培育基金（WK2016Z10）；皖南医学院中青年基金（WK201507）；安徽省大学生创新创业训练项目（201710368116，201610368065，201510368064） |
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| 摘 要： | 目的：本研究旨在探索金叶女贞果实花青素对完全弗氏佐剂（CFA）诱导慢性炎性痛的镇痛作用及其可能的中枢机制。方法：雄性SD大鼠30只随机分为三组（n=10）：正常对照组、慢性炎性痛模型组（左后足跖注射100 μl CFA）、花青素治疗组（模型+花青素90 mg·kg-1，ig，qd）。造模前和术后第1、3、5、7、9、11、13日测量各组大鼠的体重、基础痛阈（热痛阈和机械痛阈），左后肢足趾容积；术后第14日实验结束，分光光度计法测定血清各项生化指标，Western blot检测海马区总辣椒素受体（TRPV1）和磷酸化辣椒素受体（p-TRPV1）的表达。结果：花青素能提高模型组大鼠热痛阈和机械痛阈（P<0.05），降低足趾肿胀度（P<0.05），提高血清SOD水平（P<0.01），降低血清MDA和NO含量（P<0.05），降低大脑海马区p-TRPV1/TRPV1蛋白比例。结论：花青素灌胃14日处理对完全弗氏佐剂诱导的大鼠慢性炎性痛有镇痛作用，其机制可能与降低炎性因子释放，提高抗氧化能力和下调TRPV1磷酸化有关。
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| 关 键 词： | 花青素 慢性炎性痛 痛阈 辣椒素受体 |
| 收稿时间： | 2018-01-12 |
The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism |
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| WANG Jing,CHEN Ze-feng,HE Chun-xia,WANG Li-li,ZHENG Jia-hui,ZHANG Hao,WANG Zhan-lei,LU Ya-ping.The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism[J].Chinese Journal of Applied Physiology,2018,34(5):476-480. |
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| Authors: | WANG Jing CHEN Ze-feng HE Chun-xia WANG Li-li ZHENG Jia-hui ZHANG Hao WANG Zhan-lei LU Ya-ping |
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| Affiliation: | 1. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu 241002;
2. 2013 level clinical specialty, Wannan Medical College, Wuhu 241002;
3. 2015 level clinical specialty, Wannan Medical College, Wuhu 241002;
4. 2014 level anaesthetics specialty, Wannan Medical College, Wuhu 241002;
5. College of Life Science, Anhui Normal University, Wuhu 241002, China |
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| Abstract: | Objective: To investigate the effects and mechanisms of anthocyanin from Ligustrum vicaryi on chronic inflammatory pain induced by complete Freund's adjuvant. Methods: Thirty male SD rats were randomly divided into three groups (n=10):normal saline control group (NS), chronic inflammatory pain model group(Mod, injected with complete Freund's adjuvant(CFA) 100 μl to the left hind leg), anthocyanin treatment group(Ant, dosed with anthocyanins (90 m), mechanical pain threshold (MPT), and left toe volume in each group were measured before modeling and 1,3,5,7,9,11,13 days after operation. Antioxidant indexes in serum were mensurated by spectrophotometer, and the total capsaicin receptor (TRPV1) and phosphorylated capsaicin receptor (p-TRPV1) in hippocampus were detected by Western blot. Results: In comparison with controls, HPT and MPT were improved (P<0.05),toe swelling was reduced(P<0.05), the serum level of SOD was increased (P<0.01), while the levels of MDA and NO were decreased (P<0.05), the ratio of P-TRPV1/TRPV1 protein was depressed in Mod rat hippocampal region treated with anthocyanin. Conclusion: The results show that anthocyanins has an analgesic effect on chronic inflammatory pain induced by CFA, and its mechanism may be related to the improvement of antioxidant capacity and the reduction of TRPV1 phosphorylation. |
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| Keywords: | anthocyanins chronic inflammatory pain pain threshold TRPV1 |
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