| 孕康口服液对胚胎着床障碍大鼠的安胎作用 |
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| 引用本文： | 郑祥,郭林峰,戴明珠,吕圭源,陈素红.孕康口服液对胚胎着床障碍大鼠的安胎作用[J].中国应用生理学杂志,2018,34(6):543-547. |
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| 作者姓名： | 郑祥 郭林峰 戴明珠 吕圭源 陈素红 |
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| 作者单位： | 1. 温州医科大学药学院, 浙江 温州 325035;
2. 浙江中医药大学现代中药与健康产品研究所, 杭州 310053;
3. 浙江工业大学长三角绿色制药协同创新中心, 杭州 310014 |
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| 基金项目： | 浙江省重点实验室（2012E10002） |
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| 摘 要： | 目的：通过建立胚胎着床障碍致流产模型，探讨已上市中成药孕康口服液的安胎作用。方法：将妊娠大鼠分为6组：正常对照组（NC），模型组（MG），地屈孕酮组（DT），孕康口服液低、中、高3个剂量组（YK-L、YK-M、YK-H），每组11只。自妊娠第1日起，每天灌胃给药，DT组灌胃剂量为3.02 mg/kg，孕康口服液各组灌胃剂量分别为4、6、9 ml/kg，NC组、MG组给予等体积的纯化水，连续10 d。妊娠第3日，除NC组外，其余各组按5 mg/kg体质量颈背部皮下注射米非司酮造成胚胎着床障碍模型。妊娠第10日，各组腹主动脉采血，酶联免疫法测定血清卵泡刺激素（FSH）、干扰素-γ（IFN-γ）、白介素（IL-4）；取连胎子宫观察胚胎着床数目，HE染色观察子宫病理变化。结果：与NC组相比，MG组胚胎着床数目和血清FSH、IL-4水平均显著降低（P<0.05，0.01），并出现子宫腺上皮增生、腺腔内炎性细胞浸润等病理变化。与MG组相比，YK-M、YK-H组均能显著性升高胚胎着床数目和血清FSH、IL-4水平（P<0.05，0.01）；孕康口服液各剂量组均能明显改善模型大鼠子宫腺上皮增生、腺腔内炎性细胞浸润等病理变化。各组间血清IFN-γ水平无明显差异。结论：孕康口服液可能通过升高胚胎着床障碍大鼠血清性激素FSH和免疫细胞因子IL-4水平，改善子宫内膜病理变化，提高胚胎着床数目，从而发挥安胎作用。
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| 关 键 词： | 孕康口服液 胚胎着床障碍 安胎 卵泡刺激素 白介素-4 大鼠 |
| 收稿时间： | 2018-03-20 |
Effects of Yun Kang oral liquid on rats model of embryo implantation disorder |
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| ZHENG Xiang,GUO Lin-feng,DAI Ming-zhu,LYU Gui-yuan,CHEN Su-hong.Effects of Yun Kang oral liquid on rats model of embryo implantation disorder[J].Chinese Journal of Applied Physiology,2018,34(6):543-547. |
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| Authors: | ZHENG Xiang GUO Lin-feng DAI Ming-zhu LYU Gui-yuan CHEN Su-hong |
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| Affiliation: | 1. College of Pharmacy, Wenzhou Medical University, Wenzhou 325035;
2. Modern Chinese Medicine and Health Products Research Institute, Zhejiang Chinese Medical University, Hangzhou 310053;
3. Collaborative Innovation Center of Yangtze Delta Region Green Pharmaceuticals, Zhejiang Technology University, Hangzhou 310014, China |
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| Abstract: | Objective: Through the establishment of abortion model caused by embryo implantation difficulties, exploring the role of Yun Kang oral liquid in protecting embryos.Methods: The pregnant rats were divided into 6 groups:normal control group (NC), model group (MG), dydrogesterone group (DT), and three dose groups of low, medium and high levels of Yun Kang oral liquid (YK-L, YK-M, YK-H), 11 in each group.From the first day of pregnancy, daily intragastric administration, the dose of DT group was 3.02 mg/kg, and the doses of Yun Kang oral liquid were 4, 6, and 9 ml/kg, respectively.The rats in NC and MG were treated with an equal volume of purified water for 10 days.On the third day of pregnancy, except for the NC group, the other groups were injected with mifepristone subcutaneously at the back of the neck at a dose of 5 mg/kg to cause an embryo implantation barrier model.On the 10th day of pregnancy, blood was collected from the abdominal aorta in each group.Serum follicle stimulating hormone (FSH), interferon-γ (IFN-γ) and interleukin (IL-4) were measured by enzyme-linked immunosorbent assay.The number of embryo implantation was observed in the uterus, and the pathological changes of the uterus were observed by HE staining.Results: Compared with the NC group, the number of embryo implantation and the serum levels of FSH and IL-4 in the MG group were decreased significantly (P< 0.05, 0.01), and pathological changes such as uterine glandular epithelial hyperplasia and inflammatory cell infiltration in the glandular cavity were observed.Compared with MG group, the number of embryo implantation and serum FSH and IL-4 levels of rats in YK-M and YK-H groups were increased significantly (P<0.05, 0.01).The pathological changes such as uterine glandular epithelial hyperplasia and inflammatory cell infiltration in the gland were also improved.There was no significant difference in serum IFN-γ levels between the groups.Conclusion: Yun Kang oral liquid may improve the endometrial pathological changes and increase the number of embryo implantation by increasing the levels of serum sex hormone FSH and immune cytokine IL-4 in embryo implantation impediment rats. |
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| Keywords: | Yun Kang oral liquid embryo implantation disorder tocolysis FSH IL-4 rats |
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