| 灵芝蛛网病病原菌及其生物学特性 |
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| 引用本文： | 许蓉,刘正慧,付永平,李玉.灵芝蛛网病病原菌及其生物学特性[J].菌物学报,2019,38(5):669-678. |
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| 作者姓名： | 许蓉 刘正慧 付永平 李玉 |
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| 作者单位： | 吉林农业大学食药用菌教育部工程研究中心 吉林长春130118;吉林农业大学食药用菌教育部工程研究中心 吉林长春130118;吉林农业大学食药用菌教育部工程研究中心 吉林长春130118;吉林农业大学食药用菌教育部工程研究中心 吉林长春130118 |
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| 基金项目： | 公益性行业（农业）科研专项(201503137);菌类作物优质高产抗病种质资源挖掘、创制及应用学科创新引智基地（“111”基地）(D17014) |
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| 摘 要： | 为明确吉林省蛟河市灵芝Ganoderma lingzhi栽培主产区发生的疑似灵芝蛛网病的病原菌,作者通过罹病灵芝子实体病原物的分离纯化、致病性测定、形态学和分子生物学鉴定以及病原菌的生物学特性研究,证明引起吉林省蛟河市灵芝蛛网病的病原菌为嗜菌枝葡霉Cladobotryum mycophilum。该菌营养体最适生长条件为温度25℃、pH 5、蔗糖作碳源、酵母浸粉作氮源,光照对菌丝体生长有一定的抑制作用,完全黑暗最适宜生长。本文研究结果为进一步研究该病害的发生规律和防治措施提供了理论参考。
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| 关 键 词： | 灵芝 蛛网病 形态学 ITS序列 生物学特性 |
| 收稿时间： | 2018-12-24 |
Identification and biological characteristics of Cladobotryum mycophilum causing cobweb disease on Ganoderma lingzhi |
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| Authors: | XU Rong LIU Zheng-Hui FU Yong-Ping LI Yu |
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| Affiliation: | Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China |
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| Abstract: | In 2018, the alleged cobweb disease was found in Ganoderma lingzhi-growing farm at Jiaohe, Jilin Province. Morphological identification and molecular analysis of ITS DNA sequence proved that the pathogen was Cladobotryum mycophilum. Artificial inoculation confirmed that the pathogen infected fruitbodies of Ganoderma lingzhi. Its mycelia grew favorably under the conditions of 25°C and pH 5 on medium using glucose as carbon source and yeast extract as nitrogen source. Light might inhibit mycelial growth to some extent, while dark stimulated mycelial growth. The results provide a useful reference for effective control of the cobweb disease. |
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| Keywords: | Ganoderma lingzhi cobweb disease morphology ITS sequence biological characteristics |
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