| 六妹羊肚菌多糖的提取工艺优化及结构表征和抗氧化活性 |
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| 引用本文： | 王珍珍,官月,刘洋,刘淑艳.六妹羊肚菌多糖的提取工艺优化及结构表征和抗氧化活性[J].菌物学报,2019,38(9):1548-1558. |
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| 作者姓名： | 王珍珍 官月 刘洋 刘淑艳 |
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| 作者单位： | 吉林农业大学植物保护学院 吉林长春130118;吉林农业大学植物保护学院 吉林长春130118;吉林农业大学食药用菌教育部工程研究中心 吉林长春130118 |
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| 基金项目： | 国家重点研发计划政府间国际科技合作重点专项(2018YFE0107800);吉林省教育厅科学技术研究项目(JJKH20180676KJ) |
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| 摘 要： | 六妹羊肚菌Morchella sextelata是一种珍贵的食药用真菌,具有重要的经济价值。本研究在单因素试验基础上,采用响应面分析法优化其多糖提取工艺,利用Sevage法及透析法对粗多糖进行初步纯化,获得六妹羊肚菌多糖（Morchella sextelata polysaccharide,MSP）组分。采用凝胶色谱-示差-多角度激光光散射（gel permeation chromatography- refractive index-multi angle laser light scattering,GPC-RI-MALLS）、高效阴离子交换色谱（high performance anion exchange chromatography,HPAEC）、傅里叶变换红外光谱仪（fourier transform infrared spectrometer,FTIR）和气相色谱质谱联用仪（gas chromatography-mass spectrometry,GC-MS）等对其进行结构分析,并检测了该多糖清除自由基活性的能力。结果表明,六妹羊肚菌多糖最优提取工艺为：提取温度89.94℃、液料比31.07mL/g、提取时间162.86min。在此工艺条件下,提取率为23.98%。该多糖主要由分子量为4.655×10 6Da和6.571×10 4Da的两个组分组成,其单糖组成为葡萄糖、甘露糖、半乳糖,摩尔百分比分别为71.60%、23.70%和4.70%。该多糖的糖苷键主要有T-Glc、1,2-Man、1,4-Glc、1,6-Man、1,3,4-Man、1,4,6-Gal。此外,该多糖具有较强的清除2,2‐联氮基双‐3‐乙基苯并噻唑啉‐6‐磺酸2,2‐azino bis (3‐ethylbenzothiazoline‐6‐sulfonic acid),ABTS]、1,1‐二苯基‐2‐苦基肼（1,1‐diphenyl‐2‐picrylhydrazyl,DPPH）和羟自由基活性的能力。本研究结果为六妹羊肚菌多糖功能食品的开发和利用提供研究基础。
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| 关 键 词： | 羊肚菌 多糖 响应面分析 结构分析 自由基清除 |
| 收稿时间： | 2019-03-15 |
Extraction process optimization,structural characterization and antioxidant activities of polysaccharide from Morchella sextelata |
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| Authors: | WANG Zhen-Zhen GUAN Yue LIU Yang LIU Shu-Yan |
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| Affiliation: | 1. College of Plant Protection, Jilin Agricultural University, Changchun, Jilin 130118, China3. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China |
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| Abstract: | Morchella sextelata is a precious edible and medicinal fungus with important economic value. Based on single factor experiment, Box-Behnken design and response surface methodology were used to optimize the extraction process of polysaccharide. The crude polysaccharide was purified by Sevage method and dialysis, and component MSP (Morchella sextelata polysaccharide) was obtained. The structure of MSP was analyzed by gel permeation chromatography-refractive index-multi angle laser light scattering (GPC-RI-MALLS), high performance anion exchange chromatography (HPAEC), fourier transform infrared spectrometer (FTIR) and gas chromatography-mass spectrometry (GS-MS). The free radical scavenging activities of MSP also were determined. The results indicated that the optimum extraction conditions included extraction temperature of 89.94°C, ratio of extracting solution to raw material of 31.07mL/g and extraction time of 162.86min. Under the optimized conditions, the polysaccharide extraction rate was 23.98%. MSP was mainly composed of two components with molecular weight of 4.655×10 6Da and 6.571×10 4Da, and its monosaccharides were mainly glucose, mannose, and galactose with mole percentages of 71.60%, 23.70% and 4.70% respectively. The main glycoside bonds are T-Glc, 1,2-Man, 1,4-Glc, 1,6-Man, 1,3,4-Man, and 1,4,6-Gal. The polysaccharide MSP possessed strong scavenging ability to 2,2‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) free radicals, 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radicals and hydroxyl radicals. |
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| Keywords: | Morchella polysaccharide response surface analysis structural analysis radical-scavenging |
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