| 响应面法优化细脚棒束孢中N6-(2-羟乙基)腺苷的超声提取工艺研究 |
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| 引用本文： | 长城,秦立武,于文杰,刘凤霞,吴秀玲,王群,范宇光.响应面法优化细脚棒束孢中N6-(2-羟乙基)腺苷的超声提取工艺研究[J].菌物学报,2019,38(9):1559-1569. |
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| 作者姓名： | 长城 秦立武 于文杰 刘凤霞 吴秀玲 王群 范宇光 |
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| 作者单位： | 长白山科学研究院长白山生物群落与生物多样性吉林省联合重点实验室 吉林二道白河 133613;海南医学院海南省药用植物研究开发重点实验室 海南海口571199 |
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| 基金项目： | 长白山科学研究院开放基金(2016007);长白山科学研究院开放基金(201805);吉林省中医药科技项目(2019176);国家自然科学基金(31400024);国家自然科学基金(31860009);吉林省优秀青年人才基金(20180520035JH) |
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| 摘 要： | 为提高细脚棒束孢中N 6-(2-羟乙基)腺苷（简称HEA）的提取效率,在超声时间、提取液pH、料液比3个单因素试验的基础上,运用响应面法对超声提取细脚棒束孢中HEA工艺进行优化。结果表明,液料比对提取效果的影响最大,其次是提取液pH,超声时间因素影响最小。响应面法优化后,细脚棒束孢的最佳超声提取工艺为：超声时间31min、液料比22:1（mL/g）、pH 4.88,Box-Behnken模型预测值为0.86mg/g,实际值为(0.86+0.03)mg/g,二者偏差为0.10%,说明响应面优化超声提取细脚棒束孢中HEA工艺稳定可行。该研究所得提取工艺适用于不同虫草类真菌腺苷类成分的提取及分析,具有省时高效、节约能源的优势,可用于虫草的质量评价及开发等研究。
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| 关 键 词： | 超声时间 液料比 高效液相色谱 二极管阵列检测器 工艺 |
| 收稿时间： | 2019-03-27 |
Optimization of ultrasound extraction of N~6-(2-hydroxyethyl)-adenosine from Isaria tenuipes using response surface methodology |
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| Authors: | CHANG Cheng QIN Li-Wu YU Wen-Jie LIU Feng-Xia WU Xiu-Ling WANG Qun FAN Yu-Guang |
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| Affiliation: | 1. Changbai Mountain Academy of Sciences, Jilin Provincial Joint Key Laboratory of Changbai Mountains Biocoenosis & Biodiversity, Erdaobaihe, Jilin 133613, China;2. Hainan Medical University, Hainan Provincial Key laboratory of R & D on Tropical Medicinal Herbs, Haikou, Hainan 571199, China; |
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| Abstract: | Response surface methodology was used to optimize ultrasonic extraction process of N 6-(2-hydroxyethyl) adenosine (HEA) in Isaria tenuipes on the basis of three factors, namely extraction time, pH value and liquid-to-solid ratio. The results showed that liquid-to-solid ratio had priority over pH value in influencing the yield of HEA product, while extraction time was the least influential. The optimum ultrasonic extraction parameters were as follows: ultrasonic duration of 31min, liquid-to-solid ratio of 22:1 (g/mL), pH value of 4.88, and Box-Behnken model predicted value of 0.86mg/g. The actual value of Box-Behnken model is (0.86+0.03)mg/g, with deviation of 0.10%. The method was stable and feasible for the extraction and analysis of adenosine from different Cordyceps s.l. samples, showing its advantages of time saving, high efficiency and energy saving. The method is useful for quality evaluation and product development of Cordyceps and related fungi. |
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| Keywords: | utrasound time liquid-to-solid ratio high performance liquid chromatography diode array detector process |
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