Organellar proteomics: turning inventories into insights |
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| Authors: | Andersen Jens S Mann Matthias |
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| Affiliation: | Centre for Experimental Bioinformatics, CEBI, University of Southern Denmark, Campusvej 55, DK-5230 Odense, Denmark. jens.andersen@bmb.sdu.dk |
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| Abstract: | Subcellular organization is yielding to large-scale analysis. Researchers are now applying robust mass-spectrometry-based proteomics methods to obtain an inventory of biochemically isolated organelles that contain hundreds of proteins. High-resolution methods allow accurate protein identification, and novel algorithms can distinguish genuine from co-purifying components. Organellar proteomes have been analysed by bioinformatic methods and integrated with other large-scale data sets. The dynamics of organelles can also be studied by quantitative proteomics, which offers powerful methods that are complementary to fluorescence-based microscopy. Here, we review the emerging trends in this rapidly expanding area and discuss the role of organellar proteomics in the context of functional genomics and systems biology. |
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