microRNA‐577 suppresses tumor growth and enhances chemosensitivity in colorectal cancer |
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| Authors: | Haiping Jiang Hui Ju Lin Zhang Haijun Lu Kan Jie |
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| Affiliation: | 1. Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China;2. Department of Gastroenterology, Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China;3. Department of Medical Oncology, Qinghai People's Hospital, Xining, People's Republic of China |
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| Abstract: | In this work, we aimed to determine the expression and biological functions of microRNA (miR)‐577 in colorectal cancer (CRC). The results showed that miR‐577 was downregulated in CRC specimens and cell lines. Restoration of miR‐577 significantly suppressed the proliferation and colony formation and induced a G0/G1 cell cycle arrest in CRC cells. 5‐Fluorouracil (5‐FU)‐resistant SW480 cells (SW480/5‐FU) were found to have elevated levels of miR‐577. Ectopic expression of miR‐577 enhanced 5‐FU sensitivity in SW480/5‐FU cells. Heat shock protein 27 (HSP27) was identified as a target gene of miR‐577. Enforced expression of HSP27 reversed the effects of miR‐577 on CRC cell growth and 5‐FU sensitivity. Xenograft tumors derived from miR‐577‐overexpressing SW480 cells exhibited significantly slower growth than control tumors. In conclusion, our results support that miR‐577 acts as a tumor suppressor in CRC likely through targeting HSP27. Therefore, miR‐577 may have therapeutic potential in the treatment of CRC. |
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| Keywords: | colorectal cancer drug resistance growth HSP27 miR‐577 |
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