| 利用精子介导法向蚕卵导入外源基因的研究 |
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| 引用本文： | 郭秀洋,周泽扬,冯丽春,汪琳,鲁成,向仲怀.利用精子介导法向蚕卵导入外源基因的研究[J].生物化学与生物物理进展,2001,28(3):423-425. |
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| 作者姓名： | 郭秀洋 周泽扬 冯丽春 汪琳 鲁成 向仲怀 |
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| 作者单位： | 西南农业大学 |
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| 基金项目： | 重庆市科委院士基金(渝科委计［1998］19号)和中华农业科教人才基金(99-03-A-1)资助. |
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| 摘 要： | 为建立家蚕转基因中切实可行、操作简便的外源基因导入方法，进行了精子介导法探索，以精子介导法的三种方式向家蚕导入所构建质粒pFbGFP，并通过PCR扩增和DNA印迹等手段，已连续两代从基因组DNA检测到导入外源基因GFP的存在，其中的一种导入方式到第二代阳性率约30% .结果表明该法可有效进行家蚕转基因的外源基因导入.
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| 关 键 词： | 转基因，家蚕，精子介导 |
| 收稿时间： | 6/5/2000 12:00:00 AM |
| 修稿时间： | 7/7/2000 12:00:00 AM |
Sperm-mediated Gene Transformation of Silkworm |
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| GUO Xiu-Yang,ZHOU Ze-Yang,FENG Li-Chun,WANG Lin,LU Cheng and XIANG Zhong-Huai.Sperm-mediated Gene Transformation of Silkworm[J].Progress In Biochemistry and Biophysics,2001,28(3):423-425. |
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| Authors: | GUO Xiu-Yang ZHOU Ze-Yang FENG Li-Chun WANG Lin LU Cheng and XIANG Zhong-Huai |
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| Affiliation: | The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China;The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China;The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China;The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China;The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China;The Key Sericulture Laboratory of Agricultural Ministry, College of Sericulture &Silk, Southwest Agricultural University,Chongqing 400716,China |
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| Abstract: | To find practical and simple transformation methods in transgenic silkworm research, the sperm-mediated methods were tried. The constructed plasmid pFbGFP was transformed into silkworm(Bombyx mori) with three different sperm-mediated transformation methods. Positive results of PCR and Southern blotting was screened in the following two generations. 30% positive rate of the second generation was obtained by one of the methods. This result showed that this was an effective method for transformation of silkworm with foreign gene. |
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| Keywords: | sperm-mediating silkworm(Bombyx mori) transgenic |
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