| NGAL基因在永生化食管上皮细胞恶性转化中过表达的研究 |
| |
| 引用本文： | 许丽艳,李恩民,熊华淇,蔡唯佳,沈忠英.NGAL基因在永生化食管上皮细胞恶性转化中过表达的研究[J].生物化学与生物物理进展,2001,28(6):839-843. |
| |
| 作者姓名： | 许丽艳 李恩民 熊华淇 蔡唯佳 沈忠英 |
| |
| 作者单位： | 1. 汕头大学医学院肿瘤病理研究室,汕头,515031
2. 汕头大学医学院生物化学与分子生物学教研室,汕头,515031 |
| |
| 基金项目： | 国家自然科学基金青年基金项目（39900069）. |
| |
| 摘 要： | 为研究NGAL（neutrophil gelatinase-associated lipocalin）基因在永生化食管上皮细胞恶性转化中的表达情况，以永生化食管上皮细胞系SHEE和食管癌细胞系SHEEC互为对照，用cDNA微列阵进行筛选，用RNA印迹和RT-PCR进行鉴定，cDNA克隆测序后与GenBank进行BLAST分析比较.结果表明NGAL基因在SHEEC中出现显著差异过表达，其cDNA序列与小鼠24p3、大鼠NRL（neu-related lipocalin）、人中性粒细胞NGAL和卵巢癌NGAL具有较高的相似性.这提示NGAL基因在永生化食管上皮细胞恶性转化中可能发挥着重要作用，可能是一种新的癌基因或促癌基因.
|
| 关 键 词： | NGAL，cDNA微列阵，差异表达基因，食管癌 |
| 收稿时间： | 2001/1/20 0:00:00 |
| 修稿时间： | 2001年1月20日 |
Study of Neutrophil Gelatinase-assiciated Lipocalin(NGAL) Gene Overexpression in the Progress of Malignant Transformation of Human Immortalized Esophageal Epithelial Cell |
| |
| XU Li-Yan,LI En-Min,XIONG Hua-Qi,CAI Wei-Jia and SHEN Zhong-Ying.Study of Neutrophil Gelatinase-assiciated Lipocalin(NGAL) Gene Overexpression in the Progress of Malignant Transformation of Human Immortalized Esophageal Epithelial Cell[J].Progress In Biochemistry and Biophysics,2001,28(6):839-843. |
| |
| Authors: | XU Li-Yan LI En-Min XIONG Hua-Qi CAI Wei-Jia and SHEN Zhong-Ying |
| |
| Affiliation: | Institute of Oncologic Pathology, Medical College of Shantou University, Shantou 515031, China;Department of Biochemistry and Molecular Biology,Medical College of Shantou University, Shantou 515031, China;Institute of Oncologic Pathology, Medical College of Shantou University, Shantou 515031, China;Institute of Oncologic Pathology, Medical College of Shantou University, Shantou 515031, China;Institute of Oncologic Pathology, Medical College of Shantou University, Shantou 515031, China |
| |
| Abstract: | In order to study the neutrophil gelatinase-assiciated lipocalin(NGAL)gene expression character in the progress of malignant transformation of human immortalized esophageal epithelial cell, differentially expressed NGAL gene was identified by using cDNA microarray in the human immortalized esophageal epithelial cell line(SHEE) and malignant transformed esophageal cancer cell line(SHEEC). NGAL expression profile was further confirmed by Northern blot and RT-PCR. A cDNA encoding NGAL from SHEEC was amplified by PCR and sequenced. Alignment was analyzed by NCBI database. The results indicated that NGAL gene was overexpressed in the SHEEC. The coding region cDNA of NGAL from SHEEC was cloned and identified. Alignment of its deduced amino acid sequence compared to the mouse 24p3 protein, the rat neu-related lipocalin(NRL), the human NGAL from neutrophil and ovarian cancer demonstrated a very high degree of conservation. It can be concluded that NGAL overexpression possibly played an important role in the progress of malignant transformation of human immortalized esophageal epithelial cell. NGAL may be a new oncogene or promoter-tumor gene. |
| |
| Keywords: | neutrophil gelatinase-assiciated lipocalin(NGAL) cDNA microarray differentially expressed gene esophageal cancer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
