| 基于PC/Linux的核酸序列分析系统的构建及其应用 |
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| 引用本文： | 张成岗,欧阳曙光,张绍文,瞿祥虎,鱼咏涛,周钢桥,吴松锋,贺福初.基于PC/Linux的核酸序列分析系统的构建及其应用[J].生物化学与生物物理进展,2001,28(2):263-266. |
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| 作者姓名： | 张成岗 欧阳曙光 张绍文 瞿祥虎 鱼咏涛 周钢桥 吴松锋 贺福初 |
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| 作者单位： | 军事医学科学院放射医学研究所基因组学与蛋白质组学研究室, 北京 100850 |
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| 基金项目： | 军事医学科学院科技创新研究启动基金(9905105)，国家“863”项目(863-102-10-04-04)、国家杰出青年科学基金(39620514)与国家自然科学基金重点项目(39730310)部分资助. |
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| 摘 要： | 基于PC机和Linux操作系统, 利用Phred/Phrap/Consed软件和Blast软件, 构建了核酸序列大规模自动分析系统. 该套系统可自动完成从测序峰图向核酸序列的转化、载体序列去除、序列自动拼接、重复序列鉴定以及序列的相似性分析, 可加速对大规模测序数据的分析和利用.
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| 关 键 词： | PC机 Linux操作系统 Phred/Phrap/Consed软件 Blast软件 生物信息学 大规模测序 |
| 收稿时间： | 3/6/2000 12:00:00 AM |
| 修稿时间： | 2000年3月6日 |
Construction and Application of a Large-scale DNA Sequence Analysis System Based on PC/Linux |
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| ZHANG Cheng-Gang,OUYANG Shu-Guang,ZHANG Shao-Wen,QU Xiang-Hu,YU Yong-Tao,ZHOU Gang-Qiao,WU Song-Feng and HE Fu-Chu.Construction and Application of a Large-scale DNA Sequence Analysis System Based on PC/Linux[J].Progress In Biochemistry and Biophysics,2001,28(2):263-266. |
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| Authors: | ZHANG Cheng-Gang OUYANG Shu-Guang ZHANG Shao-Wen QU Xiang-Hu YU Yong-Tao ZHOU Gang-Qiao WU Song-Feng and HE Fu-Chu |
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| Affiliation: | Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China;Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract: | More and more DNA sequences have been obtained since the start-up of human genome project. Powerful system is badly needed for data mining on these DNA sequences. Based on a personal computer and Linux operating system, the Phred/Phrap/Consed software and Blast software were used to construct a platform for batch analysis of the sequences, including identifying raw DNA sequence from chromatogram file, vector sequence removing, contig analysis (sequence assembly), repeat sequence identifying and sequence similarity analysis. Result demonstrated that this robust platform could accelerate data analysis for large-scale DNA sequencing. |
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| Keywords: | personal computer Linux operating system Phred/Phrap/Consed software Blast software bioinformatics large-scale DNA sequencing |
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