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豚鼠齿状回颗粒细胞在追踪性眨眼条件反应巩固过程中的活动
引用本文:胡 波,林 曦,熊 雁,黄 维,刘国龙,隋建峰.豚鼠齿状回颗粒细胞在追踪性眨眼条件反应巩固过程中的活动[J].生物化学与生物物理进展,2006,33(11):1051-1060.
作者姓名:胡 波  林 曦  熊 雁  黄 维  刘国龙  隋建峰
作者单位:1. 第三军医大学基础医学部生理学教研室,重庆,400038
2. 第三军医大学高原医学系中心实验室,重庆,400038
摘    要:海马在追踪性眨眼条件反应的巩固过程中发挥重要作用,但解剖学上与其紧密联系的齿状回在此过程中的作用尚不清楚. 实验拟观察齿状回颗粒细胞在追踪性眨眼条件反应巩固过程中的放电活动,阐明齿状回在此海马依赖任务中所发挥的作用. 条件反射组动物 (n=8) 首先接受 200 ms 声音条件刺激,间隔 600 ms 后,再被给予 200 ms 吹气非条件刺激,多次重复配对,建立追踪性眨眼条件反应. 对照组动物 (n=8) 接受非配对出现的上述两种刺激. 采用在体单细胞外记录技术,研究习得条件反应豚鼠的齿状回颗粒细胞在条件反应巩固过程中的放电活动. 结果显示:a. 通过 14 天的训练,条件反射组动物均建立了追踪性眨眼条件反应,而非配对组动物则没有建立该条件反应;b. 齿状回颗粒细胞在追踪性眨眼条件反应的巩固过程中表现出不同的活动模式,如在声音条件刺激、间隔期或吹气非条件刺激出现后活动的增强. 这些结果提示:齿状回可能参与巩固追踪性眨眼条件反应所需的神经环路,其颗粒细胞在追踪性眨眼条件反应巩固过程中可能编码不同的信息.

关 键 词:齿状回  颗粒细胞  追踪性条件反射  眨眼  单电极记录  豚鼠
收稿时间:5/9/2006 12:25:02 AM
修稿时间:6/23/2006 9:32:47 PM

Activity of Single Granule Cell in the Dentate Gyrus of Guinea Pig During The Consolidation of Trace Eyeblink Conditioned Responses
HU Bo,LIN Xi,XIONG Yan,HUANG Wei,LIU Guo-Long and SUI Jian-Feng.Activity of Single Granule Cell in the Dentate Gyrus of Guinea Pig During The Consolidation of Trace Eyeblink Conditioned Responses[J].Progress In Biochemistry and Biophysics,2006,33(11):1051-1060.
Authors:HU Bo  LIN Xi  XIONG Yan  HUANG Wei  LIU Guo-Long and SUI Jian-Feng
Affiliation:Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China;Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China;Central Laboratory, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China;Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China;Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China;Department of Physiology, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China
Abstract:The hippocampus plays a critical role during the consolidation of trace eyeblink conditioned responses (CRs). However, the role of its related structure such as dentate gyrus (DG) remains unclear. The present study was aimed at monitoring the activity of single granule cell in the DG during the consolidation of trace eyeblink CRs, and elucidating the possible role of DG during this hippocampus-dependent task. Guinea pigs (n=8) were trained on a trace eyeblink conditioning paradigm using a 200-ms tone conditioned stimulus (CS), a 200-ms corneal airpuff unconditioned stimulus (US) and a 600-ms trace interval. Controls consisted of pseudo- conditioned guinea pigs (n=8). Extracellular single unit recordings in vivo were performed in the DG of learner animals during the consolidation of trace eyeblink CRs. The results revealed that all the trace-conditioned animals acquired the trace eyeblink CRs over 14 training days, however, none of the pseudo-conditioned animals did. Furthermore, 23 of 40 single granule cells in the DG of learner animals exhibited heterogeneous activity patterns during the consolidation of trace eyeblink CRs such as increases in activities to the tone CS, trace interval or airpuff US. The results suggested that the DG might participate in the neural circuit important for the consolidation of trace eyeblink CRs, and that the granule cells might encode different information during the consolidation of trace eyeblink CRs.
Keywords:dentate gyrus  granule cell  trace conditioning  eyeblink  single unit recording  guinea pig
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