| 大鼠不同发育时期胰腺相关蛋白的差异表达 |
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| 引用本文： | 周锦勇,胡静静,仲燕,刘超,柴伟栋,袁栎,滕丽萍,德伟.大鼠不同发育时期胰腺相关蛋白的差异表达[J].生物化学与生物物理进展,2004,31(12):1106-1113. |
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| 作者姓名： | 周锦勇 胡静静 仲燕 刘超 柴伟栋 袁栎 滕丽萍 德伟 |
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| 作者单位： | 1. 江苏省生殖医学重点实验室,南京医科大学生物化学与分子生物学系,南京,210029
2. 江苏省人民医院内分泌科,南京,210029 |
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| 基金项目： | 江苏省科委应用基础资助项目（BZ2001046）. |
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| 摘 要： | 探讨大鼠胰腺不同发育时期相关蛋白的差异表达,应用显微技术分离了大鼠孕15.5天，孕18.5天胚胎胰腺和新生鼠及成年鼠的胰腺，提取其蛋白质后，用固相pH梯度双向聚丙烯酰胺凝胶电泳和质谱分析等蛋白质组学方法，得到了4个不同发育时期的蛋白质表达谱.对其中的6个在孕18.5天胚胎胰腺中有高丰度表达，而在成年鼠胰腺中缺失的蛋白质点，4个在成年胰腺中特异表达的蛋白质点, 8个在成年胰腺中表达明显下调的蛋白质点和1个在成年中表达上调的点，进行了肽质量指纹分析和蛋白质鉴定，共获得18个点的肽质量指纹图.经BIOWORK等软件搜索大鼠非冗余蛋白质数据库来鉴定其身份，发现其中7个点为大鼠甲胎蛋白（AFP）、5个点为胰脂酶相关蛋白1前体、1个点为微管蛋白β、2个点为蛋白二硫异构酶、1个为FLN29基因产物的类似物、1个为胰蛋白酶V-A前体、1个为过氧化物氧化还原酶4.其中AFP为特异表达于大鼠胚胎期及新生期胰腺的蛋白质，在孕18.5天的胰腺中表达量最高，在成年胰腺中极低表达.对它们的功能和与胚胎胰腺代谢调节功能完善过程的可能关系进行了初步探讨.
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| 关 键 词： | 大鼠胰腺，发育，双向凝胶电泳，质谱分析，肽质量指纹图，蛋白质组学 |
| 收稿时间： | 2004/7/28 0:00:00 |
| 修稿时间： | 2004/9/30 0:00:00 |
Differentially Expressed Rat Pancreas Protein Profiles in The Different Developmental Stages |
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| ZHOU Jin-Yong,HU Jing-Jing,ZHONG Yan,LIU Chao,CAI Wei-Dong,YUAN Li,TENG Li-Ping and DE Wei.Differentially Expressed Rat Pancreas Protein Profiles in The Different Developmental Stages[J].Progress In Biochemistry and Biophysics,2004,31(12):1106-1113. |
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| Authors: | ZHOU Jin-Yong HU Jing-Jing ZHONG Yan LIU Chao CAI Wei-Dong YUAN Li TENG Li-Ping and DE Wei |
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| Affiliation: | Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;The Endocrine Department of The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;The Endocrine Department of The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China;Jiangsu Province Key Laboratory of Reproductive Medicine, Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | In order to reveal proteins differentially expressed in the process of rat pancreas development, two-dimensional gel electrophoresis and mass spectrometry were performed on rat pancreas at different stages. After rat pancreas microseperated, protein extracted from of four stages: 15.5 embryonic days (E15.5), E18.5, newborn and adult were separated by immobilized pH gradient two-dimensional gel electrophoresis. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS)and ESI-MS were performed with the proteins which were found to be significantly different presented in these four maps, 8 of which were down-expressed and 1 of which up-expressed at the adult stage, 4 of which specially expressed at adult stage while 6 of which specially expressed in E18.5 stage. All together 18 protein spots were identified. 7 spots were identified as alpha fetoprotein (AFP). 5 spots were pancreatic lipase related protein 1 precursor. 1 spot was tublin beta 5.2 spots were protein disulfide isomerase. The other 3 spots were similar to FLN29 gene product, Trypsin V-A precursor, and Peroxiredoxin 4.Among them, AFP specially expressed in new born and E18.5 stage. The development of pancreas gave rise to the changes of the protein expression patterns. This helps to understand the molecular mechanisms of rat embryonic pancreatic development. |
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| Keywords: | rat pancreas development two-dimensional gel electrophoresis mass spectrometry peptide mass fingerprinting proteomics |
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