| 酵母基因中断技术 |
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| 引用本文： | 徐志刚,霍克克,李育阳.酵母基因中断技术[J].生物化学与生物物理进展,2001,28(2):176-180. |
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| 作者姓名： | 徐志刚 霍克克 李育阳 |
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| 作者单位： | 复旦大学生命科学学院，遗传工程国家重点实验室，上海 200433 |
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| 基金项目： | 国家863计划资助项目(863-102-11-02-02). |
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| 摘 要： | 酵母基因中断技术是研究酵母基因功能的重要手段，自80年代初诞生以来经历了不断的改进和发展.PCR介导的酵母基因中断技术，大大简化了操作，实现了酵母基因的精确缺失；酵母基因的多重中断技术，可在酵母内实现多个基因的中断；可进行大规模基因中断和功能分析的酵母基因中断技术，适应了在酵母全基因组测序完成的情况下进行功能基因组学研究的要求.酵母基因中断技术对人类基因功能研究也有很大启示作用.
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| 关 键 词： | 酵母，基因中断，功能基因组学 |
| 收稿时间： | 2000/4/25 0:00:00 |
| 修稿时间： | 6/7/2000 12:00:00 AM |
Gene Disruption in Yeast |
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| XU Zhi-Gang,HUO Ke-Ke and LI Yu-Yang.Gene Disruption in Yeast[J].Progress In Biochemistry and Biophysics,2001,28(2):176-180. |
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| Authors: | XU Zhi-Gang HUO Ke-Ke and LI Yu-Yang |
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| Affiliation: | State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China;State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China;State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China |
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| Abstract: | Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast. Since 1980’s, it has been deve loped a lot. PCR-mediated gene disruption technique makes the manipulation easi er and it can be used to precisely delete genes in yeast. Multi-gene disruption technique can delete several genes successively in the same yeast strain. After the completion of the yeast Saccharomyces cerevisiae genome sequencing, the gene disruption technique for systematic analysis meets the need of the functio nal genomics in yeast. It also enlighten the study on human functional genomics. |
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| Keywords: | yeast gene disruption functional genomics |
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