| 检测人成纤维细胞增殖的XTT比色法 |
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| 引用本文： | 沈雁,刘锡麟,唐毅,钟灿灿,梁佩红,李斯明.检测人成纤维细胞增殖的XTT比色法[J].生物化学与生物物理进展,2002,29(4):659-662. |
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| 作者姓名： | 沈雁 刘锡麟 唐毅 钟灿灿 梁佩红 李斯明 |
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| 作者单位： | 广州市红十字会医院,广州市创伤外科研究所,广州,510220 |
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| 摘 要： | 利用3种不同来源的成纤维细胞(大鼠肾上皮细胞,人胎肺成纤维细胞,人成纤维细胞)的活细胞线粒体脱氢酶在电子偶联剂硫酸酚嗪甲酯（phenazine methosulfate, PMS）的协同作用下，还原四氮唑复合物(XTT)形成可溶性的棕黄色甲簪（formazan）产物，测定细胞甲簪的生成量来反映细胞的生长与活性状态，并与传统的四甲基偶氮唑盐(MTT)方法作比较.结果表明，XTT方法直接测定水溶性的甲簪产物，敏感度高于MTT法，具有操作简单、快速、灵敏度高、结果准确的优点，为成纤维细胞的研究建立了新的检测方法.
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| 关 键 词： | 成纤维细胞 细胞培养，增殖 |
| 收稿时间： | 1/8/2002 12:00:00 AM |
| 修稿时间： | 2002年1月8日 |
XTT Colorimetric Method for Detecting Cellular Growth of Human Fibroblasts |
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| SHEN Yan,LIU XI-Lin,TANG Yi,ZHONG Can-Can,LIANG Pei-Hong and LI Si-Ming.XTT Colorimetric Method for Detecting Cellular Growth of Human Fibroblasts[J].Progress In Biochemistry and Biophysics,2002,29(4):659-662. |
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| Authors: | SHEN Yan LIU XI-Lin TANG Yi ZHONG Can-Can LIANG Pei-Hong and LI Si-Ming |
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| Affiliation: | Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China;Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China;Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China;Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China;Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China;Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, China |
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| Abstract: | In order to establish a sensitive method for detecting cellular growth of fibroblasts. Three kinds of fibroblasts were used, including normal rat kidney (NRK), human embryo lung fibroblast cell (MRC 5) and normal human fibroblast(NF). In those cells, mitochondrion dehydrogenase together with phenazine methosulfate (PMS) reduces 2,3 bis (2 methoxy 4 nitro 5 sulfophenyl) 5 (phenylamino) carbonyl] 2H tetrazolium hydroxide (XTT) and a water soluble and light brown formazan product is formed. Cell growth and viability were obtained by measuring the metabolic product and compared with the traditional 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) method. It was shown that three kinds of cells have different metabolic response to XTT. XTT method directly detected water soluble formazan product. Its sensitivity was higher than MTT. These results indicate that it is a simple, rapid, sensitive and stable method. XTT colorimetric method establishes a new detective method for keloid research of traumatic surgery. |
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| Keywords: | fibroblast cell culture proliferation |
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