Quantification of the Pyrrolizidine Alkaloid Jacobine in Crassocephalum crepidioides by Cation Exchange High‐Performance Liquid Chromatography |
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| Authors: | Wilfried Rozhon Lukas Kammermeier Sebastian Schramm Nayeem Towfique N Adebimpe Adedeji S Adesola Ajayi Brigitte Poppenberger |
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| Affiliation: | 1. Biotechnology of Horticultural Crops, Technische Universit?t München, Freising‐Weihenstephan, Germany;2. Department of Crop Production and Protection, Obafemi Awolowo University, Nigeria |
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| Abstract: | Introduction Pyrrolizidine alkaloids (PAs) are secondary plant metabolites with considerable hepatoxic, tumorigenic and genotoxic potential. For separation, reversed phase chromatography is commonly used because of its excellent compatibility with detection by mass spectrometry. However, reversed phase chromatography has a low selectivity for PAs. Objective The objective of this work was to investigate the suitability of cation exchange chromatography for separation of PAs and to develop a rapid method for quantification of jacobine in Crassocephalum crepidioides that is suitable for analysis of huge sample numbers as required for mutant screening procedures. Results We demonstrate that cation exchange chromatography offers excellent selectivity for PAs allowing their separation from most other plant metabolites. Due to the high selectivity, plant extracts can be directly analysed after simple sample preparation. Detection with UV at 200 nm instead of mass spectrometry can be applied, which makes the method very simple and cost‐effective. The recovery rate of the method exceeded 95%, the intra‐day and inter‐day standard deviations were below 7% and the limit of detection and quantification were 1 mg/kg and 3 mg/kg, respectively. Conclusion The developed method is sufficiently sensitive for reproducible detection of jacobine in C. crepidioides. Simple sample preparation and rapid separation allows for quantification of jacobine in plant material in a high‐throughput manner. Thus, the method is suitable for genetic screenings and may be applicable for other plant species, for instance Jacobaea maritima. In addition, our results show that C. crepidioides cannot be considered safe for human consumption. Copyright © 2017 John Wiley & Sons, Ltd. |
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| Keywords: | cation exchange chromatography Crassocephalum crepidioides HPLC jacobine pyrrolizidine alkaloids |
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