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连作土灭菌对葡萄根际土壤微生物群落功能的影响
引用本文:李坤,朱绍坤,郭修武,谢洪刚,郭印山,刘镇东.连作土灭菌对葡萄根际土壤微生物群落功能的影响[J].生态学杂志,2016,35(4):972-976.
作者姓名:李坤  朱绍坤  郭修武  谢洪刚  郭印山  刘镇东
作者单位:(;1.沈阳农业大学园艺学院, 沈阳 110866; ;辽宁经济管理干部学院, 沈阳 110112)
基金项目:现代农业产业技术体系专项基金项目(CARS-30-yz-06);国家自然科学基金项目(31101503);辽宁省科技厅重大科技攻关项目(2011204001);教育部高校博士点专项资金项目(20112103120012)资助
摘    要:In order to explore the correlation between soil microbial community function and plant growth, using 30 year continuous cropping soil of grapevines as research object, we studied the effects of sterilization of continuous cropping soil at different temperatures on the growth of grapevines and the microbial community function of rhizosphere soil. The results showed that plant height and stem diameter of grapevines grown in continuous cropping soil were lower than those in the other treatments. With the increasing sterilization temperature, the plant height and stem dia meter of grapevines increased. The ratio of bacteria to fungi in rhizosphere soil increased with the increasing sterilization temperature. The activity of rhizosphere microbes using carbon source was in order of continuous cropping soil sterilized at 100 ℃ > non continuous cropping soil > continuous cropping soil sterilized at 60 ℃ > continuous cropping soil. The regression analysis showed that amino acid (carbon source) in Biolog ECO plate had a significant correlation with microbial metabolic activity of rhizosphere soil. PCA analysis showed that lysine in root exudate had a highest contribution to the variance of principal components in each treatment, and it may play an important role in the obstacle of continuous cropping of grapevines.

关 键 词:根际微生物  种群结构  代谢活性  根系分泌物  氨基酸
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