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滨海湿地植物芦苇可培养内生真菌的多样性
引用本文:李海林,马斌,张晓黎,张倩倩,郭晓红,范德朋,龚骏.滨海湿地植物芦苇可培养内生真菌的多样性[J].生态学杂志,2016,27(7):2066-2074.
作者姓名:李海林  马斌  张晓黎  张倩倩  郭晓红  范德朋  龚骏
作者单位:1.中国科学院烟台海岸带研究所微型生物生态与物质循环实验室, 山东烟台 264003;;2.中国科学院大学, 北京 100049;;3.佛山市碧沃丰生物科技股份有限公司, 广东佛山 528200
基金项目:本文由国家自然科学基金青年基金项目(41301333)、烟台市科技发展计划项目(2014ZH073,2015ZH074)和烟台市双百人才计划项目资助
摘    要:内生真菌在植物生长以及抵御环境胁迫过程中起着非常重要的生态作用.本研究从黄河三角洲滨海湿地1350个芦苇组织切片中分离得到318株内生真菌,通过对rDNA ITS的分型、测序及系统进化分析,研究了该地区芦苇植株根、茎、叶中可培养内生真菌的种类组成及在不同盐度、不同组织中的分布情况.根据序列相似性(以98%为阈值),共获得12个真菌分类操作单元(OTUs).在门级分类水平上,子囊菌门为绝对优势菌群, 在各组织和站位中均有分布.芦苇根组织中分离得到的内生真菌OTU数相对较多,叶组织和茎组织中分离到的OTU数相同,且叶中的OTU在根中均存在.潮上区(低盐区)内生真菌OTU数最多,高潮区次之.根中可培养内生真菌的丰富度和多样性指数最高,叶中多样性最低;潮上区丰富度最高,中潮区多样性最高.尽管不同盐度梯度及不同芦苇组织中都有其特异的菌株,但总体看,土壤盐度及不同组织对可培养内生真菌的种类组成的影响并不显著(ANOSIM,P>0.05).链格孢属是所有样品共有的优势菌.

关 键 词:滨海湿地  芦苇  内生真菌  盐度胁迫
收稿时间:2015-11-25

Diversity of culturable endophytic fungi of common reed (Phragmites australis) in coastal wetland.
LI Hai-lin,MA Bin,ZHANG Xiao-li,ZHANG Qian-qian,GUO Xiao-hong,FAN De-peng,GONG Jun.Diversity of culturable endophytic fungi of common reed (Phragmites australis) in coastal wetland.[J].Chinese Journal of Ecology,2016,27(7):2066-2074.
Authors:LI Hai-lin  MA Bin  ZHANG Xiao-li  ZHANG Qian-qian  GUO Xiao-hong  FAN De-peng  GONG Jun
Affiliation:1.Laboratory of Coastal Microbial Ecology and Matter Cycles, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong, China;;2.University of Chinese Academy of Sciences, Beijing 100049, China;;3.Foshan Bio-Form Technology Co., Ltd., Foshan 528200, Guangdong, China
Abstract:Endophytic fungi play an important ecological role in promoting growth and alleviating environmental stress of host plants. In this study, we investigated the diversity and community composition of endophytic fungi isolated from the common reed in wetlands of the Yellow River Delta. A total of 318 endophytic fungal isolates were obtained from 1350 tissue (leaf, stem, and root) fragments and from three tidal zones with different soil salinity levels. rDNA ITS regions of these isolates were PCR amplified, sequenced, and phylogenetically analyzed. All the sequences were divided into 12 operational taxonomic units (OTUs) at the 98% similarity threshold. Ascomycota was found to be the most abundant flora at the level of the phylum and distributed in all tissues and sites. There were generally richer OTUs in roots than in leaves and stems, and leaves and stems shared the same OTU numbers. All the OTUs retrieved from leaves appeared in roots. The supra tide (low salinity zone) had the most OTUs among the three sampling sites, followed by high tide. Furthermore, the roots had the highest richness and diversity, whereas the leaves had the lowest; supra tide held the highest richness, and the middle tide had the highest diversity. Although unique OTUs could be found in different tissues and tidal zones of different salinity, the communities of culturable endophytic fungi were not substantially different among tissues or tidal zones (ANOSIM, P>0.05). Alternaria sp. was the shared dominant taxa among all samples.
Keywords:coastal wetland  Phragmites australis  endophytic fungi  salt stress  
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