Isolation and characterization of tri‐ and tetra‐repeat microsatellite loci in the white‐spotted charr Salvelinus leucomaenis (Salmonidae) |
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| Authors: | S Yamamoto M Sekino |
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| Affiliation: | 1. National Research Institute of Aquaculture, Fisheries Research Agency, Nikko, Tochigi 321‐1661, Japan;2. National Research Institute of Fisheries Science, Fisheries Research Agency, Fuku‐ura, Kanazawa, Yokohama, Kanagawa 236‐8648, Japan |
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| Abstract: | Tri‐ and tetra‐motif repeat microsatellite marker loci were developed for the white‐spotted charr Salvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite‐primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180–0·600 and 0·188–0·599, respectively. |
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| Keywords: | 454 pyrosequencing next‐generation sequencer polymorphism short tandem repeat simple sequence repeat |
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