| 两株中和性抗hTNFa单抗可变区与hTNFa相互作用的计算机模拟及实验研究 |
| |
| 引用本文： | 陈建军,孙苗,方佳,刘惠,陈常庆,王德宝.两株中和性抗hTNFa单抗可变区与hTNFa相互作用的计算机模拟及实验研究[J].生物工程学报,2000,16(1):36-41. |
| |
| 作者姓名： | 陈建军 孙苗 方佳 刘惠 陈常庆 王德宝 |
| |
| 作者单位： | 1. 中国科学院上海生物工程研究中心,上海,200233
2. 中国科学院上海生物化学研究所,上海,200031 |
| |
| 基金项目： | 国家高技术研究与发展(九·五)计划项目(103-13-01-01)
。 |
| |
| 摘 要： | 在ＳＧＩ图形工作站上，用同源蛋白结构预测的方法，建立了２－株中和性抗ｈＴＮＦα小鼠单抗（１Ｃ３Ｅ６，３Ｆ６Ａ１０）可变区的三结构模型，并在实验研究２株单抗表位表异性的基础上，根据２株单抗与ｈＴＮＦα分子表面的形状、静电性、疏水性、氢键等性质，对２株单抗可变区与ｈＴＮＦα分子的可能结合模式进行了模拟和分析，再根据结合模型，设计并制备了２个ｈＴＮＦα突变体，然后从实验与计算机模拟两方面着手，对比研究了
|
| 关 键 词： | hTNFα 单克隆抗体 三维结构模型 分子间结合位 |
| 文章编号： | 1000-3061(2000)01-0036-06 |
| 修稿时间： | 1999-01-04 |
Computer Modeling and Experimental Research of Interactions between Two Anti-hTNFa Monoclonal Antibody Variable Regions and hTNFa |
| |
| CHEN Jian-jun,SUN Miao,FANG Jia,LIU Hui,CHEN Chang-qing,WANG De-bao.Computer Modeling and Experimental Research of Interactions between Two Anti-hTNFa Monoclonal Antibody Variable Regions and hTNFa[J].Chinese Journal of Biotechnology,2000,16(1):36-41. |
| |
| Authors: | CHEN Jian-jun SUN Miao FANG Jia LIU Hui CHEN Chang-qing WANG De-bao |
| |
| Affiliation: | CHEN Jian-jun , SUN Miao ,FANG Jia
,LIU Hui ,CHEN Chang-qing ;
(Shanghai Research Centre of Bilotechnology, The Chinese Academy of Sciences, Shanghai
200233);
WANG De-bao ;
(Shanghai lnstitute of Biochemistry, The Chinese Academy of Sciences, Shanghai 200031) |
| |
| Abstract: | On SGI workstation, we constructed two anti-hTNF alpha McAbs by means of homologous protein-structure-prediction method. And then, on the basis of relative experimental results and the surface properties of hTNF alpha and two McAbs, we performed the docking of hTNF alpha into two anti-hTNF alpha McAbs. In order to confirm the models, we prepared two hTNF alpha mutants designed according to the binding models, analysed and predicted the possible changes in complexes resulted from hTNF alpha mutations. The experimental analysis results proved these complex models. This will make the base of our next antibody humanization and/or reshape work. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
|
点击此处可从《生物工程学报》下载全文 |
|
