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磁修饰酵母对直接大红染料的生物吸附
引用本文:吴琦,单志,沈茂,李双江,陈惠.磁修饰酵母对直接大红染料的生物吸附[J].生物工程学报,2009,25(10):1477-1482.
作者姓名:吴琦  单志  沈茂  李双江  陈惠
作者单位:四川农业大学生命科学与理学院,雅安,625014
摘    要:本研究采用水相合成的纳米级铁磁流体对灭活酿酒酵母细胞进行磁修饰,获得了具有良好磁响应的酵母。傅立叶变换红外光谱分析表明,该修饰酵母在Fe-O特征峰581cm1处的吸收明显加强。透射电镜观察表明,纳米磁性颗粒单个或成团聚集在酵母细胞表面。在本实验条件下,160μL磁修饰酵母对1mL浓度为0.4mg/mL直接大红染料吸附率达100%;8min达到吸附平衡;在70%乙醇中,染料脱吸附率为99.18%。由于磁修饰酵母吸附力强,吸附速度快,易于磁分离,是一种有前景的水溶性染料生物吸附剂。

关 键 词:酿酒酵母  磁修饰  直接大红  生物吸附  
收稿时间:2009/3/30 0:00:00

Biosorption of direct scarlet dye on magnetically modified Saccharomyces cerevisiae cells
Qi Wu,Zhi Shan,Mao Shen,Shuangjiang Li and Hui Chen.Biosorption of direct scarlet dye on magnetically modified Saccharomyces cerevisiae cells[J].Chinese Journal of Biotechnology,2009,25(10):1477-1482.
Authors:Qi Wu  Zhi Shan  Mao Shen  Shuangjiang Li and Hui Chen
Abstract:We prepared the nano-sized magnetic fluid in aqueous phase solution to modify the killed yeast cells (Saccharomyces cerevisiae). Subsequently, the yeast cells with good magnetic response was obtained.The infrared spectroscopy analysis showed that the modified yeast cells increased obviously the absorption at the Fe-O characteristic peak of 581 cm?1. Moreover, the transmission electron microscopy picture indicated the magnetic nanoparticles presented individually or aggregately on the yeast cell surface. Und...
Keywords:Saccharomyces cerevisiae  magnetic modification  direct scarlet  biosorption  
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