| 苏云金芽胞杆菌幕虫亚种晶胞粘连现象与晶体蛋白基因cry26Aa所在质粒有关 |
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| 引用本文： | 鞠守勇,纪芳,朱自敏,喻子牛,孙明,汤治国,张睿.苏云金芽胞杆菌幕虫亚种晶胞粘连现象与晶体蛋白基因cry26Aa所在质粒有关[J].微生物学报,2007,47(1). |
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| 作者姓名： | 鞠守勇 纪芳 朱自敏 喻子牛 孙明 汤治国 张睿 |
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| 作者单位： | 华中农业大学生命科学技术学院,农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划) |
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| 摘 要： | 苏云金芽胞杆菌幕虫亚种T02菌株的伴胞晶体在芽胞外壁内侧形成,呈现晶胞粘连的现象。在此菌株中克隆了cry26 Aa和cry28 Aa两个基因,并对晶胞粘连现象与质粒的相关性做了系统研究。通过消除幕虫亚种T02菌株的质粒,得到了仅消除cry26 Aa所在质粒的菌株BMB1151和无质粒的菌株BMB1152。通过穿梭载体将cry26 Aa和cry28 Aa两个基因分别和同时转化无质粒突变株BMB1152并表达,形成的晶体与芽胞独立存在不能粘连,表明在幕虫亚种染色体背景下仅仅cry的表达不能形成晶胞粘连现象,从而推断晶胞粘连现象可能与幕虫亚种两个基因所在的质粒有关;进一步的研究发现将cry26 Aa在仅消除cry26 Aa所在质粒的突变株BMB1151中表达,形成的晶体与芽胞也分别独立存在不能粘连,从而进一步推断幕虫亚种晶胞粘连现象与cry26 Aa所在质粒有关。
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| 关 键 词： | 苏云金芽胞杆菌 幕虫亚种 晶胞粘连 |
The plasmid harboring cry26Aa may contribute to the phenomenon of spore-crystal connection in Bacillus thuringiensis subsp. finitimus |
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| JV Shou-yong,JI Fang,ZHU Zi-min,YU Zi-niu,SUN Ming,TANG Zhi-guo,ZHANG Rui.The plasmid harboring cry26Aa may contribute to the phenomenon of spore-crystal connection in Bacillus thuringiensis subsp. finitimus[J].Acta Microbiologica Sinica,2007,47(1). |
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| Authors: | JV Shou-yong JI Fang ZHU Zi-min YU Zi-niu SUN Ming TANG Zhi-guo ZHANG Rui |
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| Abstract: | Parasporal crystal in B. thuringiensis subsp. finitimus T02 forms inside the exosporium and remains attached to the spore after mother cell lysis. Two crystal protein gene cry26 Aa and cry28 Aa were cloned from this strain. The relationship between the phenomenon of spore-crystal connection and the plasmids was investigated in this work. Mutants BMB1151 curing of the plasmid harboring cry26 Aa, and BMB1152 curing of all plasmids were obtained from B. thuringiensis subsp. finitimus T02.The spore-crystal connection didn't appeared when cry26 Aa and cry28 Aa were transformed back into the strain BMB1152 by shuffle vector alone or in combination together. It suggested the plasmids of B. thuringiensis subsp. finitimus T02 may contribute to the phenomenon. When gene cry26 Aa was transformed back into in the strain BMB1151, spore-crystal connection didn't form either. So the plasmid harboring cry 26 Aa may also contribute to the phenomenon of spore-crystal connection in B. thuringiensis subsp. finitimus T02. |
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| Keywords: | cry26Aa cry28Aa |
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