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葡萄糖对表皮葡萄球菌生物被膜形成的影响及调节机制的研究
引用本文:靳嘉巍,张力,查锡良,李华林,瞿涤.葡萄糖对表皮葡萄球菌生物被膜形成的影响及调节机制的研究[J].微生物学报,2005,45(3):431-436.
作者姓名:靳嘉巍  张力  查锡良  李华林  瞿涤
作者单位:1. 复旦大学上海医学院生物化学与分子生物学系卫生部糖复合物重点实验室,上海,200032
2. 复旦大学上海医学院生物化学与分子生物学系教育部医学分子病毒学重点实验室,上海,200032
基金项目:国家“8 63计划”( 2 0 0 1AA2 2 3 0 11)~~
摘    要:生物被膜(Biofilm)是条件致病菌表皮葡萄球菌(Staphylococcusepidermidis)的主要致病因素,生物被膜的形成依赖多糖PIA合成,合成PIA的糖基转移酶由icaADBC基因编码。以生物被膜形成能力不同的菌株为对象,通过研究不同环境对生物被膜形成、细菌总糖量及相关基因表达的变化,探索外界环境对生物被膜形成的影响及葡萄糖对生物被膜诱导的分子机制。有利于生物被膜形成培养条件促进生物被膜形成及多糖的表达,葡萄糖能诱导ica基因的表达和生物被膜形成,ica基因的反义寡核苷酸(ODN)能对抗葡萄糖的作用;葡萄糖作用下不同生长周期生物被膜形成相关基因ica、icaR、AtlE表达不同。表皮葡萄球菌生物被膜的形成与细菌糖代谢有关,葡萄糖通过上调ica表达诱导生物膜形成,但不需要ica基因的持续表达;葡萄糖的诱导作用不是直接通过调节AtlE和icaR基因来实现的

关 键 词:表皮葡萄球菌  生物被膜  多聚β-1  6-2脱氧-2-氨基-D-吡喃葡萄糖(PIA)  反义寡核苷酸(ODN)  ica  AtlE
文章编号:0001-6209(2005)03-0431-06
修稿时间:2004年10月8日

Effect of glucose on bofilm and the gene ica expression in Staphylococcus epidermidis with different biofilm-forming capability
JIN Jia-wei,ZHANG Li,ZHA Xi-Liang,LI Hua-lin,QU Di.Effect of glucose on bofilm and the gene ica expression in Staphylococcus epidermidis with different biofilm-forming capability[J].Acta Microbiologica Sinica,2005,45(3):431-436.
Authors:JIN Jia-wei  ZHANG Li  ZHA Xi-Liang  LI Hua-lin  QU Di
Affiliation:Key Laboratory of Glycoconyugate Research of Ministry of Public Health, Fudan University, Shanghai 200032, China. jwjin@shmu.edu.cn
Abstract:The infection of \%S. epidermidis,\% an opportunistic human pathogen, depends on biofilm formation, and biofilm formation is closely related to environment. Researches in the thesis focused on two strains of \%S. epidermidis\% with different capability of biofilm formation. To find the mechanism of response to environment on biofilm formation, biofilm formation and expression of \%ica, icaR, AtlE\% in theses \%S. epidermidis\% cultivated in different grow environment and in media with glucose for different time were assayed. Glucose can induce the biofilm formation by inducing \%ica\% gene, but the inducing do not need continued \%ica\% expression, and other genes also contribute to the regulation; anti-ODN specially binding \%icaADBC\% can withstand biofilm inducing from glucose. These results suggest that biofilm formation closely related to growth environment, which is a complex regulation mechanism. Biofilm formation is closely related to bacteria energy metabolism and cell wall synthesis. Some crucial factors in the complex and integrated regulation system have not been known yet.
Keywords:Staphylococcus epidermidis  Biofilm  Polysaccharide intercellular adhesion (PIA)  ODN  AtlE  ica
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