| 胡枝了属根瘤菌的多相分类研究 |
| |
| 引用本文： | 姚竹云,陈文新.胡枝了属根瘤菌的多相分类研究[J].微生物学报,1999,39(4):287-295. |
| |
| 作者姓名： | 姚竹云 陈文新 |
| |
| 摘 要： | 采用数值分类，全细胞可溶性蛋白电泳分析，ＤＮＡ，Ｇ＋Ｃｍｏｌ％和ＤＮＡ相关性的测定以及１６ＳｒＤＮＡＰＣＲ－ＲＦＬ分析等多相分类技术对来源于不同地区的１６种寄主的胡枝子根瘤菌进行了系统的分类研究，数值分类的结果表明，在６７％的相似性水平上，全部供试菌可以为快生型根瘤菌和慢性型根瘤菌两大群，在８０％的相似性水平上又可分为两个亚群。在此基础上，对各亚群的胡枝子根瘤菌进行了ＤＮＡ相关性的测定，以进一步证
|
| 关 键 词： | 胡枝子 根瘤菌 多相分类 数值分类 DNA相关性 |
Study on polyphasic taxonomy of rhizobia isolated from Lespedeza species] |
| |
| Z Yao,W Chen.Study on polyphasic taxonomy of rhizobia isolated from Lespedeza species][J].Acta Microbiologica Sinica,1999,39(4):287-295. |
| |
| Authors: | Z Yao W Chen |
| |
| Affiliation: | Department of Microbiology, China Agricultural University, Beijing 100094. |
| |
| Abstract: | The diversity of rhizobia isolated from Lespedeza spp. was determined on the basis of numerical analysis of phenotypic characteristics, sodium dodecyl sulfate-polyacrylarmide gel electrophoresis (SDS-PAGE) of proteins, DNA-DNA homology and restriction fragment length polymorphism (RFLP) analysis of 16S-ribosomal DNA genes. According to numerical analysis of 125 phenotypic characteristics, strains were divided into two groups at a similarity level of 67%. Group I included all the fast-growing strains, group II included all the slow-growing strains. Above the similarity level of 80%, four subgroups could be further divided. Subgroup I was fast-growing rhizobia containing representative strain of Sinorhizobium saheli. Subgroup II, III, IV were slow-growing rhizobia. Subgroup II composed of strains isolated from Lespedeza cuneata in Beijing area and these isolates produced acid in medium containing mannitol. Subgroup III included type strain of Bradyrhizobium japonicum. The DNA G + C contents and DNA-DNA homology of the members of above four subgroups were determined. The subgroup I shared the same DNA homologous group with S. saheli, subgroup III belonged to B. japonicum, subgroup IV belonged to B. elkanii, subgroup II was an unique DNA homologous group which showed low level of DNA relatedness with other slow-growing rhizobia species. RFLP analysis of 16S rDNA genes verified that the subgroup II was a distinctive genealine and showed genetic variation within the strains in it. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《微生物学报》浏览原始摘要信息 |
|
点击此处可从《微生物学报》下载全文 |
|
