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| 红莲型水稻细胞质雄性不育花粉总蛋白质初步比较分析 | |
| 引用本文： | 文李,刘盖,王坤,彭晓珏,李国民,陶钧,朱英国.红莲型水稻细胞质雄性不育花粉总蛋白质初步比较分析[J].武汉植物学研究,2007,25(2):112-117. |
| 作者姓名： | 文李 刘盖 王坤 彭晓珏 李国民 陶钧 朱英国 |
| 作者单位： | 1. 长沙理工大学生物与食品工程学院,长沙,410076;武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072 2. 武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072 3. 长沙理工大学生物与食品工程学院,长沙,410076 |
| 基金项目： | 国家重点基础研究发展计划(973计划);中国高校博士点专项研究基金 |
| 摘 要： | 采用固相pH梯度/SDS-PAGE双向电泳对红莲型细胞质雄性不育水稻的不育系(YTA)和保持系(YTB)二核期花粉总蛋白质进行了分离,通过银染显色,获得了分辨率和重复性较好的双向电泳图谱。用PDQuest2DE软件可识别约1500个蛋白质点,其中差异表达的蛋白质点数为120。将其中15个差异点采用基质辅助激光解析电离飞行时间质谱(matrix assisted laser desorption/ionizaton time of flight mass spectrometry,MALDI-TOF-MS)进行了肽质指纹图分析,通过采用Mascot软件对MSDB数据库查询,其中7个蛋白质点得到了鉴定。YTA相对于YTB有部分参与物质和能量代谢的蛋白质缺失或表达量降低,这些蛋白质分别是水稻线粒体H -转运ATPase(H -ATPase)α链、盐诱导型膜联蛋白、线粒体NAD -依赖型苹果酶和磷酸核糖焦磷酸合成酶等。这些蛋白质的表达下调或缺失可能与线粒体提供能量不足而导致的花粉不能正常发育有关。线粒体电压依赖性阴离子通道(VDAC)这一重要蛋白质在YTA中的上调表达有可能与花粉败育过程中细胞的程序性死亡相关。 |
| 关 键 词： | 水稻 花粉 细胞质雄性不育 蛋白质组学 |
| 文章编号： | 1000-470X（2007）02-0112-06 |
| 收稿时间： | 2006-11-07 |
| 修稿时间： | 2006-11-072006-12-26 |
Preliminary Analysis of the Total Proteins of HL Type Cytoplasmic Male Sterility Rice Pollen |
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| WEN Li,LIU Gai,WANG Kun,PENG Xiao-Jue,LI Guo-Ming,TAO Jun,ZHU Ying-Guo.Preliminary Analysis of the Total Proteins of HL Type Cytoplasmic Male Sterility Rice Pollen[J].Journal of Wuhan Botanical Research,2007,25(2):112-117. | |
| Authors: | WEN Li LIU Gai WANG Kun PENG Xiao-Jue LI Guo-Ming TAO Jun ZHU Ying-Guo |
| Affiliation: | 1. College of Biological and Food Engineering, Changsha University of Science and Technology, Changsha 410076, China; 2. Key Laboratory Of MOE in Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China |
| Abstract: | The proteins of HL type cytoplasmic male sterility(CMS)rice pollen of YTA(male-sterile line)and YTB(maintenance line)were separated by two-dimensional electrophoresis with immobilized pH(3-10 non-linear,NL)gradients as the first dimension and SDS-PAGE as the second.The silver-stained proteins spots were analyzed using Image Master 2D software,there were about 1500 detectable spots on each 2D-gel,and about 120 spots were differentially expressed.With direct MALDI-TOF mass spectrometry analysis and protein database searching,7 protein spots out of 15 were identified.Among those proteins,there were 4 proteins were down-regulated or absent in YTA,which were a putative phosphoribosyl pyrophosphate synthase,a putative membrane-associated salt-inducible protein,a putative mitochondrial NAD -dependent malic enzyme and a rice mitochondrion H -transporting two-sector ATPase alpha chain respectively.It seemed reasonable to infer that the decrease or absence of those proteins in pollen might relate to the pollen sterility caused by the mitochondrial insufficient energy.While the up-regulation of the protein,mitochondrial voltage-dependent anion-selective channel(VDAC)protein,in YTA,might involve in the programmed cell death during the pollen abortion. |
| Keywords: | Rice(Oryza sativa L ) Pollen Cytoplasmic male sterility Proteomics |
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