| 中国近缘野生大麦醇溶蛋白的遗传多态性研究 |
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| 引用本文： | 唐慧慧,丁毅,胡耀军.中国近缘野生大麦醇溶蛋白的遗传多态性研究[J].武汉植物学研究,2002,20(4):251-257. |
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| 作者姓名： | 唐慧慧 丁毅 胡耀军 |
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| 作者单位： | 1. 武汉大学植物发育生物学教育部重点实验室,武汉,430072
2. 湖南湘潭师范学院 |
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| 基金项目： | 国家自然科学基金资助项目 (No.3 9670 0 5 1)，教育部骨干教师计划资助项目 |
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| 摘 要： | 随机选取来源于中国西藏24个不同行政县的181份近缘野生大科材料，其中包括47份六棱野生大麦，134份二棱野生大麦，选用青藏高原的二棱野生大麦（W2），欧洲的代表品种Betzes大麦及以色列的二棱野生大麦（Is)作对照。利用A-PAGE（Acidic-polyacrylamide gel electrophoresis)法进行了醇溶蛋白遗传多态性的研究。结果表明，184份供试材料共得到60种不同的电泳图谱，说明西藏近缘野生大麦醇溶蛋白遗传多态性非常丰富，其中有38种图谱为单一材料所独有，以ZYM0019和ZYM1488材料为代表的2种醇溶蛋白图谱占供试材料的29.3%，在西藏分布较为普遍，聚类分析表明地理环境相似的地区有着相似的图谱类型，图谱类型与地理生态环境具有一定的相关性。讨论了中国近缘野生大麦醇溶蛋白的多态性与地理分布的关系。以及栽培大麦的起源中心等问题。
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| 关 键 词： | 醇溶蛋白图谱 中国近缘野生大麦 遗传多态性 |
| 文章编号： | 1000-470X(2002)04-0251-07 |
| 收稿时间： | 2001-12-11 |
| 修稿时间： | 2002-1-25 |
Genetic Polymorphism of Hordein in Wild Relatives of Barley from China |
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| TANG Hui-Hui,DING Yi,HU Yao-Jun.Genetic Polymorphism of Hordein in Wild Relatives of Barley from China[J].Journal of Wuhan Botanical Research,2002,20(4):251-257. |
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| Authors: | TANG Hui-Hui DING Yi HU Yao-Jun |
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| Affiliation: | Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China |
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| Abstract: | Using A PAGE,genetic polymorphism of hordein was analyzed among 181 wild relatives of barley from 24 different counties in Tibet,including 47 six rowed wild relatives of barley and 134 two rowed wild forms.Three barleys that came from Qinghai Province of China,Europe and Israel respectively were used as controls.The hordein patterns showed a very large variation.60 types of hordein patterns were obtained from the tested materials and 38 materials had their unique patterns respectively. The results revealed that 184 materials were clustered into 3 major groups.One major group had only two materials.Two types of hordein patterns that were represented by ZYM0019 and ZYM1488 were widespread and abundant in Tibet,and about 29 3 percent of the tested materials had these patterns.The findings suggested a correlation between hodein patterns and geographic environment.Furthermore,the origin of cultivated barley was discussed. |
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| Keywords: | Hordein pattern Wild relatives of barley from China Genetic polymorphism |
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