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| 濒危植物矮沙冬青的等位酶多样性及居群分化 | |
| 引用本文： | 陈国庆,黄宏文,葛学军.濒危植物矮沙冬青的等位酶多样性及居群分化[J].武汉植物学研究,2005,23(2):131-137. |
| 作者姓名： | 陈国庆 黄宏文 葛学军 |
| 作者单位： | 1. 中国科学院华南植物园,广州,510650;中国科学院研究生院,北京,100039 2. 中国科学院武汉植物园,武汉,430074 3. 中国科学院华南植物园,广州,510650 |
| 基金项目： | 中国科学院知识创新工程重要方向项目(KSCX2-SW-104)，国家自然科学基金资助项目(30370282)。 |
| 摘 要： | 采用超薄平板微型聚丙烯酰胺等电聚焦技术，对珍稀濒危植物矮沙冬青(Ammopiptanthus nanus)6个自然居群的等位酶遗传多样性进行了初步研究。8个酶系统、l9个酶位点的检测结果表明，矮沙冬青具有较低的遗传变异水平。物种水平上的遗传多样性参数为：P=21．05％，A=1．21，Ht=0．040；居群水平上的平均遗传多样性为：P=14．04％，A=1．14(0．04)，Ht=0．036(0．018)，比长寿命多年生木本的狭域分布种和特有种的多样性水平都低。F-统计量分析显示，矮沙冬青在居群水平(F15=0．129)和总居群水平(FIT=0．202)上都表现为杂合子缺乏；Fsr=0．085表明居群问遗传分化较小，这与居群间较高的遗传一致度(I=0．997)的结果一致。据研究结果推测矮沙冬青进化历史上经历过遗传瓶颈，是造成遗传多样性水平偏低的重要原因。基于研究结果，提出应当重点保护遗传变异相对丰富的何依儿佣克尔(KE)居群，并且可将其作为引种的种源地。 |
| 关 键 词： | 濒危物种 矮沙冬青 等位酶 遗传多样性 居群分化 遗传瓶颈 |
| 文章编号： | 1000-470X(2005)02-0131-07 |
| 收稿时间： | 2004-8-12 |
| 修稿时间： | 2004-10-12 |
Allozyme Diversity and Population Differentiation in an Endangered Plant, Ammopiptanthus nanus (Leguminosae) |
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| CHEN Guo-Qing,HUANG Hong-Wen,GE Xue-Jun.Allozyme Diversity and Population Differentiation in an Endangered Plant, Ammopiptanthus nanus (Leguminosae)[J].Journal of Wuhan Botanical Research,2005,23(2):131-137. | |
| Authors: | CHEN Guo-Qing HUANG Hong-Wen GE Xue-Jun |
| Affiliation: | CHEN Guo-Qing~ |
| Abstract: | Ammopiptanthus nanus is a rare and endangered species, native to desert habitats in Northern China. Allozyme diversity and population differentiation were evaluated for six natural populations using isoelectric focusing in thin-layer polyacrylamide slab gels. A total of 19 loci from eight enzyme systems were detected. An extreme low level of genetic variation was revealed both at the species level(A=1.21,P=21.05%,H_e=0.040)and at population level(P=14.04%,A=1.14(0.04),H_e=0.036 (0.018) ), which were lower than those of long-lived perennial woody and narrowly distributed species. Wright's F-statistics analysis indicated a deficit of heterozygote both at population level(F_(IS)=0.129) and at the total population level (F_(IT)=0.202). A low level of genetic differentiation among populations detected (F_(ST)=0.085) was consistent with a high level of genetic identity among the populations (I= 0.997). The history of bottleneck effect was speculated to be the main course for the current genetic structure in A. nanus. Consequently, conservation concerns of this species were discussed and conservation recommendations are suggested based on the results obtained from the study. Consequently, conservation of Keyieryongker (KE) population which maintained high genetic variability is recommended and this population could act as a source of transplant. |
| Keywords: | Endangered species Ammopiptanthus nanus Allozyme Genetic diversity Population differentiation Genetic bottleneck |
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