| 从一种富含藻胆蛋白的螺旋藻中大量提取和纯化藻蓝蛋白的研究 |
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| 引用本文： | 胡一兵,胡鸿钧,李夜光,耿亚红.从一种富含藻胆蛋白的螺旋藻中大量提取和纯化藻蓝蛋白的研究[J].武汉植物学研究,2002,20(4):299-302. |
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| 作者姓名： | 胡一兵 胡鸿钧 李夜光 耿亚红 |
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| 作者单位： | 1. 中国科学院武汉植物研究所,武汉,430074;湖北民族学院生物科学与技术学院,恩施,445000
2. 中国科学院武汉植物研究所,武汉,430074 |
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| 摘 要： | 采用新鲜藻丝为原料和分段梯度盐析分离纯化钝顶螺旋藻Sp(NS)-90020的藻蓝蛋白（PC），经羟基磷灰石一次层析，能使提取的PC的纯度大于普遍认可的标准，由于该工艺流程较为简单，适合藻蓝蛋白的大量生产，藻胆蛋白经Sephadex凝胶过滤后的可达电泳纯度标准，经SDS-PAE测得PC，APC的分子量分别约为38.33kD。
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| 关 键 词： | 钝顶螺旋藻 藻蓝蛋白 别藻蓝蛋白 分离纯化 |
| 文章编号： | 1000-470X(2002)04-0299-04 |
| 收稿时间： | 2001-12-27 |
| 修稿时间： | 2002-5-9 |
Study on Mass Production of Phycobilins from PC Rich Strain of Spirulina platensis |
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| HU Yi-Bing,HU Hong-Jun,LI Ye-Guang,GENG Ya-Hong.Study on Mass Production of Phycobilins from PC Rich Strain of Spirulina platensis[J].Journal of Wuhan Botanical Research,2002,20(4):299-302. |
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| Authors: | HU Yi-Bing HU Hong-Jun LI Ye-Guang GENG Ya-Hong |
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| Affiliation: | 1. Wuhan Institute of Botany, The Chinese Academy of Sciences, Wuhan 430074, China;2. School of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, China |
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| Abstract: | Fresh algal material of the phycobilins rich strain of Spirulina platensis SP(NS 90020) was used to separate and purify PC by means of gradient salting out. Precipitates of extracts were processed by chromatographying on hydroxylapatite(HA). The procedure of extraction and purification was proved to be simple and efficient, suitable for mass production of PC. Data of SDS PAGE showed that the molecular weight of PC and APC were about 38 kD and 33 kD , respectively. |
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| Keywords: | Spirulina platensis C phycocyanin Allophycocyanin Separation and purification |
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