| 秋水仙素诱导川贝母(Fritillaria cirrhosa D.Don)愈伤组织多倍体的研究 |
| |
| 引用本文： | 王强,兰利琼,傅华龙.秋水仙素诱导川贝母(Fritillaria cirrhosa D.Don)愈伤组织多倍体的研究[J].武汉植物学研究,2002,20(6):449-452. |
| |
| 作者姓名： | 王强 兰利琼 傅华龙 |
| |
| 作者单位： | 四川大学生命科学学院,成都,610064 |
| |
| 基金项目： | 四川省科技厅应用基础项目。 |
| |
| 摘 要： | 应用组织培养技术对川贝母（Fritillaria cirrhosaD.Don)进行多倍体诱导。结果表明：组织培养条件下，将川贝母愈伤口组织在培养基中添加一定浓度的和为水仙素处理一段时间，或者经一定浓度的和为水仙素浸泡一段时间后再培养，均可诱发川贝母多倍体的产生，但以前者效果较好。在秋水仙素浓度为1000mg/L。处理5d的条件下，诱导率最高达70%。细胞染色体鉴定结果为：四倍体染色体数为2n=4x=48，而二倍体的染色体数为2n=2x=24。
|
| 关 键 词： | 川贝母 秋水仙素 多倍体 |
| 文章编号： | 1000-470X(2002)06-0449-04 |
| 收稿时间： | 2002-3-23 |
| 修稿时间： | 2002-7-26 |
Induction of Polyploid from Colchicine-Treated Fritillaria cirrhosa D.Don Callus |
| |
| WANG Qiang,LAN Li-Qiong,FU Hua-Long.Induction of Polyploid from Colchicine-Treated Fritillaria cirrhosa D.Don Callus[J].Journal of Wuhan Botanical Research,2002,20(6):449-452. |
| |
| Authors: | WANG Qiang LAN Li-Qiong FU Hua-Long |
| |
| Affiliation: | School of Life Science, Sichuan University, Chengdu 610064, China |
| |
| Abstract: | The callus of Fritillaria cirrhosa D.Don was obtained by placing the bulb on MS medium supplemented with 2 mg/L 6-BA and 1 mg/L NAA and 500 mg/L CH, and the callus was treated to get polyploid with colchicine. The result showed that polyploid callus was obtained by adding colchicine into the medium or by colchicine soaking. The former method was better than the later one. The best result for inducing tetraploid was by adding colchicine 1000 mg/L into the medium treated for 5 days, the inducing rate was up to 70%. The chromosome number of the tetraploid was 2n=4x=48, while that of diploid was 2n=2x=24. |
| |
| Keywords: | Fritillaria cirrhosa D Don Colchicine Polyploid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《武汉植物学研究》浏览原始摘要信息 |
|
点击此处可从《武汉植物学研究》下载全文 |
