| 中华水韭(Isoetes sinensis)等位酶分析的初步研究 |
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| 引用本文： | 陈媛媛,叶其刚,黄宏文.中华水韭(Isoetes sinensis)等位酶分析的初步研究[J].武汉植物学研究,2003,21(1):91-94. |
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| 作者姓名： | 陈媛媛 叶其刚 黄宏文 |
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| 作者单位： | 中国科学院武汉植物研究所/武汉植物园,武汉,430074 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目资助 ( KSCX2 -SW-10 4) |
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| 摘 要： | 采用超薄平板聚丙烯酰胺等电聚焦技术对珍稀涉危植物中华水韭（Isoetes sinensis Palmer）72个样品的等位酶遗传变异进行了初步研究。从24种酶筛选，得到了13种酶系统可用于该植物的遗传变异分析，其中7种酶系统在中华水韭的迁地保护区群中检测出16个位点，共27个等位基因，12个位点表现出多态性。遗传多样性初步分析表明：中华水韭具较低的等位酶遗传多样性。研究结果为中华水韭的综合保育及居群恢复、重建提供了初步的依据。中华水韭遗传变异的全面研究将在进一步改善等位酶方法的基础上，采用其它更为灵敏的分子标记方法做深入检测。
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| 关 键 词： | 中华水韭 凝胶电泳 等位酶分析 |
| 文章编号： | 1000-470X(2003)01-0091-04 |
| 收稿时间： | 2002-11-30 |
| 修稿时间： | 2003-1-14 |
Preliminary Study on Allozyme Analysis for Isoetes sinensis Palmer |
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| CHEN Yuan-Yuan,YE Qi-Gang,HUANG Hong-Wen.Preliminary Study on Allozyme Analysis for Isoetes sinensis Palmer[J].Journal of Wuhan Botanical Research,2003,21(1):91-94. |
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| Authors: | CHEN Yuan-Yuan YE Qi-Gang HUANG Hong-Wen |
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| Affiliation: | Wuhan Botanical Garden/Wuhan Institute of Botany, The Chinese Academy of Sciences, Wuhan 430074, China |
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| Abstract: | The allozymic diversity was preliminarily investigated for 72 samples of rare and endangered species Isoetes sinensis Palmer, using isoelectric focusing in thin-layer polyacrylamide slab gels. 24 enzyme systems were initially screened, resulting 13 workable enzyme systems. A total of 27 alleles of 16 loci were detected from 7 well-resolved enzymes. Although 12 loci were observed to be polymorphic in ex situ conserved populations at Wuhan Botanical Garden, the overall genetic diversity is low in I. sinensis. The study provided preliminary evidence that a comprehensive conservation and restoration program is needed for sustainable conserving and recovery of natural I. sinensis populations. A further study of I. sinensis population and conservation genetics is urgently needed by employing more sensitive molecular methods. |
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| Keywords: | Isoetes sinensis Palmer Gel electrophoresis Allozyme analysis |
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