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| 不结球白菜雄性不育系及其保持系花药发育的细胞学观察 | |
| 引用本文： | 朱红芳,侯瑞贤,李晓锋,朱玉英,侯喜林.不结球白菜雄性不育系及其保持系花药发育的细胞学观察[J].武汉植物学研究,2011,29(2):183-187. |
| 作者姓名： | 朱红芳 侯瑞贤 李晓锋 朱玉英 侯喜林 |
| 作者单位： | 1. 上海市农业科学院园艺研究所,上海,201106;上海市设施园艺技术重点实验室,上海,201106;南京农业大学园艺学院,南京,210095 2. 上海市农业科学院园艺研究所,上海,201106;上海市设施园艺技术重点实验室,上海,201106 3. 南京农业大学园艺学院,南京,210095 |
| 基金项目： | 上海科技攻关项目，上海绿叶蔬菜产业技术体系专项资助 |
| 摘 要： | 以不结球白菜（Brassica campestris ssp．chinensis Makino）雄性不育系及其保持系为试验材料，选择不同发育阶段的花蕾，取其花药，制成石蜡切片和超薄切片，经染色后在电子显微镜下观察。结果表明，不结球白菜雄性不育系与保持系的花药发育有明显的不同：不育系花药发育受阻于花粉母细胞分化期，形成1～3个药室，并形成正常的四分体小孢子，此时细胞组织逐步解体，形成空腔花药；最后向内皱缩；保持系花粉母细胞能形成正常的四分体，进而形成小孢子，最终形成充满正常花粉粒的花药。 |
| 关 键 词： | 不结球白菜 雄性不育 花药发育 石蜡切片 |
Cytological Observation of Anther Development between the Male Sterile Line and Its Maintainer Line in Non-heading Chinese Cabbage |
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| ZHU Hong-Fang,HOU Rui-Xian,LI Xiao-Feng,ZHU Yu-Ying,HOU Xi-Lin.Cytological Observation of Anther Development between the Male Sterile Line and Its Maintainer Line in Non-heading Chinese Cabbage[J].Journal of Wuhan Botanical Research,2011,29(2):183-187. | |
| Authors: | ZHU Hong-Fang HOU Rui-Xian LI Xiao-Feng ZHU Yu-Ying HOU Xi-Lin |
| Affiliation: | ZHU Hong-Fang1,2,3,HOU Rui-Xian1,LI Xiao-Feng1,ZHU Yu-Ying1,2*,HOU Xi-Lin3(1.Shanghai Academy of Agriculture Sciences,Shanghai 201106,China,2.Shanghai Key Lab of Protected Horticultural Technology,3.Colleoge of Horticulture,Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | Buds selected at different developmental stages of a new germplasm sterile male of non-heading Chinese cabbage and its maintainer lines were studied.Anthers were collected,made into paraffin sections,stained,and observed by electron microscopy.Results show that non-heading Chinese cabbage sterile male lines and maintainer lines exhibited quite different anther development.Male sterile lines in relation to anther development were inhibited at the stage of archesporial cell,produced only 1-3 anther sacs,and f... |
| Keywords: | Non-heading Chinese cabbage Male sterile lines Anther development Paraffin |
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