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| 烟草RAPD双引物与单引物PCR扩增多态性效率的比较分析 | |
| 引用本文： | 李延坤,陶爱芬,祁建民,周东新,王涛,张广庆,危成林,兰涛,陈顺辉.烟草RAPD双引物与单引物PCR扩增多态性效率的比较分析[J].武汉植物学研究,2010,28(3):257-262. |
| 作者姓名： | 李延坤 陶爱芬 祁建民 周东新 王涛 张广庆 危成林 兰涛 陈顺辉 |
| 作者单位： | 1. 作物遗传育种与综合利用教育部重点实验室(福建农林大学)福州,350002;山东省德州市齐河县农业局,山东齐河,251100 2. 作物遗传育种与综合利用教育部重点实验室(福建农林大学)福州,350002 3. 福建省龙岩市烟草科学研究所,福建龙岩,364000 4. 福建省南平市烟草公司,福建南平,353200 5. 福建省农业科学院作物研究所,福州,350003 6. 福建省福州市烟草科学研究所,福州,350003 |
| 摘 要： | 以两个亲缘关系较远的烟草（Nicotiana tabacum）品种台烟7号和白肋21为材料（GS=0.58）,研究了单引物扩增和双引物聚合扩增对RAPD-PCR分子标记多态性的影响。结果显示：双引物反应能够比单引物反应扩增出更多的多态性片段,双引物在白肋21和台烟7号中扩增出的多态性片段总数是单引物反应扩增出的多态性片段总数的6.6倍。因此,双引物RAPD的运用有助于提高烟草多态性分子标记的有效性。 |
| 关 键 词： | 烟草 RAPD 双引物RAPD PCR 多态性 |
Polymorphic Analysis of RAPD with Double Primers and a Single Primer in Tobacco |
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| LI Yan-Kun,TAO Ai-Fen,QI Jian-Min,ZHOU Dong-Xin,WANG Tao,ZHANG Guang-Qing,WEI Cheng-Lin,LAN Tao,CHEN Shun-Hui.Polymorphic Analysis of RAPD with Double Primers and a Single Primer in Tobacco[J].Journal of Wuhan Botanical Research,2010,28(3):257-262. | |
| Authors: | LI Yan-Kun TAO Ai-Fen QI Jian-Min ZHOU Dong-Xin WANG Tao ZHANG Guang-Qing WEI Cheng-Lin LAN Tao CHEN Shun-Hui |
| Affiliation: | 1.Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2.Bureau of Agriculture of Qihe County of Shandong Province,Qihe,Shandong 251100,China;3.Longyan Tobacco Branch Company,Longyan,Fujian 364000,China;4.Nanping Tobacco Company,Nanping,Fujian 353200,China;5.Insititute of Crop Science,Academy of Agriculture Science of Fujian Province,Fuzhou 350003,China;6.Fuzhou Institute of Tobacco Science of Fujian Province,Fuzhou 350003,China) |
| Abstract: | Two materials with a relatively wide genetic relationship（GS=0.58）,Taiyan 7 and Burley 21,were employed to study the effect of double-primers on the polymorphism of the RAPD PCR reaction,using the standard RAPD procedure（single primer） as a reference.Results showed that the double-primers reaction could amplify more polymorphic bands than the standard RAPD technique.The application of the double-primers RAPD technique could,therefore,be an effective and economical method to improve the selection efficiency of polymorphism molecular markers. |
| Keywords: | RAPD PCR |
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