| 珍稀濒危植物香果树胚性细胞悬浮系的建立和植株再生 |
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| 引用本文： | 熊丹,陈发菊,,梁宏伟,王玉兵.珍稀濒危植物香果树胚性细胞悬浮系的建立和植株再生[J].植物学报,2008,25(3):337-343. |
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| 作者姓名： | 熊丹 陈发菊 梁宏伟 王玉兵 |
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| 作者单位： | 1 三峡大学生物技术研究中心, 湖北省天然产物研究与利用重点实验室, 宜昌443002 2 湖北民族学院湖北省生物资源保护与利用重点实验室, 恩施445000 |
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| 基金项目： | 湖北省生物资源保护与利用重点实验室开放基金,国家林业局自然保护区研究中心项目,宜昌市科技局重点攻关项目 |
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| 摘 要： | 以香果树(Emmenopterys henryi)未成熟种子为试材, 探讨不同的接种量、基本培养基、糖浓度及植物生长调节物质等对体细胞胚胎生长的影响, 建立稳定的香果树胚性细胞悬浮培养与植株再生体系。结果表明: 悬浮培养条件下, 最适接种量为2%(鲜重); 较适合的基本培养基为MS; 蔗糖浓度为1%时容易使球形胚聚合体愈伤化, 浓度为3%和6%适合球形胚聚合体增殖, 浓度为9%则容易使球形胚聚合体褐化; 添加0.5 mg.L-16-BA 和0.5 mg.L-1 NAA的MS液体培养基, 当初始蔗糖浓度为3%, 然后逐步提高到6%则有利于香果树各个发育阶段的同步化; 子叶胚转到不含任何植物生长调节物质的MS固体培养基中可以长成正常植株。
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| 关 键 词： | 胚性悬浮细胞 香果树 植株再生 体细胞胚胎发生 |
| 收稿时间： | 2007-03-13 |
| 修稿时间： | 2007-11-29 |
Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi |
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| Dan Xiong,Faju Chen,Hongwei Liang,Yubing Wang.Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi[J].Bulletin of Botany,2008,25(3):337-343. |
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| Authors: | Dan Xiong Faju Chen Hongwei Liang Yubing Wang |
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| Affiliation: | 1Key Laboratory of Natural Products Research and Development of Hubei Province, Biotechnology Research Center of China Three Gorges University, Yichang 443002, China 2Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province |
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| Abstract: | Embry ogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg·L-1 6-benz ylaminopur ine (6-BA) and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg·L-1 N-acetylaspartate (NAA) and 0.5 mg·L-1 6-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg·L-1 NAA and 0.5 mg·L-1 6-BA. The optimal value of the initial cell density was 2.0% (fresh weight). Several parameters that might be as sociated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully
to the greenhouse, with a high survival rate of 95%. |
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| Keywords: | , |
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