| 白梨和砂梨S基因型确定及S34新基因的分离 |
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| 引用本文： | 曾艳玲,谭晓风,张党权,李秀根,曾晓峰.白梨和砂梨S基因型确定及S34新基因的分离[J].植物学报,2008,25(3):292-297. |
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| 作者姓名： | 曾艳玲 谭晓风 张党权 李秀根 曾晓峰 |
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| 作者单位： | 1 中南林业科技大学经济林育种与栽培国家林业局重点实验室, 长沙 410004; 2 中国农业科学院郑州果树研究所, 郑州 450009 |
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| 基金项目： | 国家林业重点科学技术研究计划林业新技术开发与储备专项,湖南省科技三项费资助项目,中南林业科技大学校科研和教改项目 |
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| 摘 要： | 梨是配子体型自交不亲和植物, 确定不同品种的S基因型是科学杂交授粉及提高梨产量和品质的基础。本文根据砂梨S1-9等位基因一级结构特征, 设计特异引物PF和PR, 以白梨(Pyrus bretschneideri)品种鹅梨(Pyrus bretschneideri ‘El i’) 和砂梨(Pyrus pyrifolia)品种博多青(Pyrus pyri folia
‘Hakataao’) 的叶片基因组DNA为模板, 通过 PCR-RFLP系统检测、克隆测
序以及生物信息学分析, 分离鉴定了它们的片段大小相似的2条S等位基因, 从中获得1条新的S基因, 命名为S34-RNase基因, 并确定了这2个梨品种的S基因型, 分别为鹅梨S13S34和博多青S22S34。
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| 关 键 词： | 分离鉴定 梨 S基因型 S34-RNase基因 |
| 收稿时间： | 2007-01-08 |
| 修稿时间： | 2007-05-29 |
Identification of S-genotypes of Pear Cultivars and Cloning of S34-allele |
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| Yanling Zeng,Xiaofeng Tan,Dangquan Zhang,Xiugen Li,Xiaofeng Zeng.Identification of S-genotypes of Pear Cultivars and Cloning of S34-allele[J].Bulletin of Botany,2008,25(3):292-297. |
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| Authors: | Yanling Zeng Xiaofeng Tan Dangquan Zhang Xiugen Li Xiaofeng Zeng |
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| Affiliation: | 1 Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China; 2 Zhengzhou Fruit Research Institute, CAAS, Zhengzhou 450009, China |
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| Abstract: | Pear is a typical fruit tree of gametophytic self-incompatibility. Identifying the S-allele genotype of different pears is an important scientific foundation for arranging varieties for pollination and cross breeding. It also establishes the foundation for inheriting and improving pear cultivars. On the basis of the reported primary structure of pear S1-S9, the primers PF and PR were designed. With the primers and leaf genomic DNA of cultivars Pyrus bretschneideri ‘Eli’ and Pyrus pyrifolia ‘Hakataao’, we us-ed PCR-RFLP,cloning, sequencing and bioinformatic analysis to isolate and identify the two S-alleles of amplification segments with similar size. One of the S-alleles was newly identified and named S34-allele. S-genotypes of the two cultivars were identified as Pyrus bretschneideri ‘Eli’ S13S34 and Pyrus pyrifoli a Hakataao’ S22S34. |
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| Keywords: | , |
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