| 刺五加叶中金丝桃苷含量的测定 |
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| 引用本文： | 曹建国,赵则海,杨逢建,李庆勇,祖元刚.刺五加叶中金丝桃苷含量的测定[J].植物学报,2005,22(2):203-206. |
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| 作者姓名： | 曹建国 赵则海 杨逢建 李庆勇 祖元刚 |
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| 作者单位： | (东北林业大学森林植物生态学教育部重点实验室 哈尔滨 150040) |
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| 基金项目： | ,教育部重点实验室基金,教育部科学技术研究项目 |
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| 摘 要： | 用高效液相色谱法测定了刺五加(Acanthopanax senticosus (Rupr.& Maxin) Harms)叶中金丝桃苷(hyperin)的含量。色谱柱为C18柱，流动相为甲醇和0.025 mol.L-1磷酸，比例为V甲醇:V0.025 mol.L-1磷酸=55:45 (混合后用三乙胺调pH3.0～3.2)，检测波长360 nm。样品用甲醇超声提取。结果表明, 金丝桃苷峰形良好，与其他组分的色谱峰达到基线分离。加样回收率为104%，相对标准偏差RSD为5.38%。本研究为刺五加叶中金丝桃苷含量的测定建立了一种可靠的方法。
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| 关 键 词： | 刺五加 金丝桃苷 高效液相色谱法 |
| 收稿时间： | 2003-12-31 |
| 修稿时间： | 2004-06-25 |
Determination of Hyperin in the Leaves of Acanthopanax senticosus |
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| CAO Jian-Guo ZHAO Ze-Hai YANG Feng-Jian LI Qing-Yong ZU Yuan-Gang.Determination of Hyperin in the Leaves of Acanthopanax senticosus[J].Bulletin of Botany,2005,22(2):203-206. |
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| Authors: | CAO Jian-Guo ZHAO Ze-Hai YANG Feng-Jian LI Qing-Yong ZU Yuan-Gang |
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| Affiliation: | (Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040) |
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| Abstract: | This paper describes the use of high performance liquid chromatography (HPLC) to determine the hyperin in the leaves of Acanthopanax senticosus on a C18 chromatogram column. Methanol:0.025 mol.L-1 phosphoric acid solution (V/V, 55:45) was used as the mobile phase (the mixture was adjusted with triethylamine to pH 3.0-3.2), and the detection wavelength was 360 nm. The sample was extracted with methanol by the ultrasonic extraction method. Hyperin content showed a symmetrical peak shape and reached the baseline separation with the peak content of other chemicals. The average recovery was 104% and the relative standard deviation (RSD) 5.38%. This study provided evidence of an accurate and credible method of determining the content of hyperin in the leaves of A. senticosus. |
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| Keywords: | , |
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