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| 猪背最长肌中肌肉生长和脂肪沉积相关基因的差异表达和主成分分析 | |
| 引用本文： | 李明洲,李学伟,朱砺,滕晓坤,肖华胜,李强,陈磊.猪背最长肌中肌肉生长和脂肪沉积相关基因的差异表达和主成分分析[J].中国生物化学与分子生物学报,2008,24(1):46-54. |
| 作者姓名： | 李明洲 李学伟 朱砺 滕晓坤 肖华胜 李强 陈磊 |
| 作者单位： | 1. 四川农业大学动物科技学院,雅安,625014 2. 生物芯片上海国家工程研究中心,上海,201203 |
| 基金项目： | 教育部长江学者和创新团队发展计划 , 高等学校博士学科点专项科研项目 , 四川省教育厅资助项目 , 四川省教育厅资助项目 |
| 摘 要： | 骨骼肌细胞和脂肪细胞在分化生长速度上相对竞争的平衡点是猪肉质和胴体性状的决定因素.利用Oligo功能分类芯片检测了瘦肉型的长白猪和脂肪型的太湖猪在初生、1、2、3、4和5月龄间背最长肌中肌肉生长和脂肪沉积相关基因的动态表达变化.差异表达分析结果显示,在初生至5月龄的品种间分别有15、16、11、13、18和20个基因的表达差异倍数大于2倍.品种内的方差分析表明,长白猪分别有18和22个基因，太湖猪分别有3和7个基因在月龄间的表达差异达极显著(Ｐ<0.01)和显著水平(Ｐ<0.05).主成分分析结果显示,先降后升是两品种内最具代表性的基因表达模式，且长白猪和太湖猪分别有7和6个基因的表达模式明显偏离其他基因，提示其可能受到了重要的调控. 此外，5个差异表达基因的荧光定量RT-PCR验证结果均与芯片结果呈正相关趋势.以上结果筛选出了对于猪肉质和胴体性状可能具有重要影响，值得深入研究的一些候选基因，为深入研究生长发育过程中参与肌纤维生长和脂肪酸合成关键基因的表达变化规律和互作调控机制提供了基础数据. |
| 关 键 词： | 猪 基因芯片 肌肉 脂肪 基因差异表达 主成分分析 |
| 收稿时间： | 2007-8-2 |
| 修稿时间： | 2007年8月2日 |
Differential Expression and Principal Component Analysis of Genes Related to Muscle Growth and Adipose Deposition |
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| LI Ming-Zhou,LI Xue-Wei,ZHU Li,TENG Xiao-Kun,XIAO Hua-Sheng,LI Qiang,CHEN Lei.Differential Expression and Principal Component Analysis of Genes Related to Muscle Growth and Adipose Deposition[J].Chinese Journal of Biochemistry and Molecular Biology,2008,24(1):46-54. | |
| Authors: | LI Ming-Zhou LI Xue-Wei ZHU Li TENG Xiao-Kun XIAO Hua-Sheng LI Qiang CHEN Lei |
| Affiliation: | College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, Sichuan, China; National Engineering Center for Biochip at Shanghai, Shanghai 201203, China) |
| Abstract: | The competitive equilibrium of growth rate of skeletal muscle cells and adipose cells determines porcine meat quality and carcass traits simultaneously. The expression changes of genes associated with muscle growth and adipose deposition in longissimus dorsi muscle at six growth stages (birth, 1, 2, 3, 4 and 5 months) of Landrace and Taihu pigs were examined by using pathway-focused Oligo microarray. The comparative results of change ratios between two pig breeds showed that the fold changes of 15, 16, 11, 13, 18 and 20 genes were over two times at same month from birth to 5 month, respectively. Variance analysis (ANOVA) revealed that differences in the expression of 18 genes in Landrace pigs and 3 genes in Taihu pigs were very significant (Ｐ<0.01) and differences for 22 genes in Landrace pigs and 7 genes in Taihu pigs were significant (Ｐ<0.05) among six growth stages. Principal component analysis (PCA) revealed that the most representative gene expression pattern of both two pig breeds represented genes which were first down-regulated strongly, and then up-regulated gradually. In addition, 7 genes of Landrace and 6 genes of Taihu pigs displayed distinctive expression pattern from other genes, which suggested that these genes maybe came under special regulation during the growth and development of skeletal muscle cells and adipose cells. Quantitative real-time RT-PCR was used to verify the microarray data for five modulated genes, and a good positive correlation between the two measures of expression was observed for both two pig breeds at different growth stages (r=0.876±0.095).These results highlight some possible candidate genes for porcine meat quality and carcass traits and provide some data on which to base further study of the expressional disciplinarian and molecular mechanism of genes related to muscle fiber growth and fatty acid biosynthesis. |
| Keywords: | pig microarray muscle adipose gene differential expression principal component analysis |
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