| MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达 |
| |
| 引用本文： | 安康,包明芳,姚宝辉,康宇坤,谭宇尘,王艳莉,苏军虎.MTNR1a和MTNR1b基因在繁殖期与非繁殖期雄性高原鼢鼠HPG轴上的表达[J].兽类学报,2022,42(4):410-419. |
| |
| 作者姓名： | 安康 包明芳 姚宝辉 康宇坤 谭宇尘 王艳莉 苏军虎 |
| |
| 作者单位： | 1.甘肃农业大学草业学院，草业生态系统教育部重点实验室，中美草地畜牧业可持续发展研究中心，兰州 7300702.甘肃农业大学—新西兰梅西大学草地生物多样性研究中心，兰州 7300703.甘肃省祁连山草原生态系统野外科学观测研究站，天祝 733200 |
| |
| 基金项目： | 国家自然科学基金(31760706)； |
| |
| 摘 要： | 高原鼢鼠 (Eospalax baileyi) 终年营地下生活，感光受洞道限制，但褪黑素 (Melatonin) 分泌水平仍存有季节差异，为探明褪黑素对高原鼢鼠季节性繁殖的调控作用，研究利用q?PCR技术检测雄性高原鼢鼠繁殖期 (5月) 和非繁殖期 (9月) 下丘脑、垂体及睾丸中褪黑素受体1a (Melatonin receptor 1a, MTNR1a) 和褪黑素受体1b (Melatonin receptor 1b, MTNR1b) 基因mRNA的相对表达量，通过免疫组织化学技术对MTNR1a和MTNR1b在睾丸中定位，并采用Image Pro Plus软件进行免疫组化阳性评价。结果发现，高原鼢鼠繁殖期下丘脑和垂体中MTNR1a基因的相对表达量显著高于非繁殖期的相对表达量 (P < 0.05)，MTNR1b基因的相对表达量在不同时期无显著差异 (P > 0.05)，但非繁殖期睾丸中MTNR1a和MTNR1b基因的相对表达量均显著高于繁殖期 (P < 0.01)；繁殖期除长形精子外的所有类型细胞以及非繁殖期的间质细胞、支持细胞和精原细胞中均观察到MTNR1a的阳性信号，繁殖期除精原细胞和长形精子细胞外的所有类型细胞，以及非繁殖期间质细胞和支持细胞中均观察到MTNR1b的阳性信号，且非繁殖期MTNR1a和MTNR1b的平均光密度值均显著高于繁殖期 (P < 0.01)。MTNR1a和MTNR1b基因在雄性高原鼢鼠HPG轴上的表达模式，提示了褪黑素在其季节性繁殖调控中的潜在作用。
|
| 关 键 词： | 高原鼢鼠 MTNR1a基因 MTNR1b基因 季节性繁殖 |
| 收稿时间： | 2021-09-02 |
Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non?breeding seasons |
| |
| Kang AN,Mingfang BAO,Baohui YAO,Yukun KANG,Yuchen TAN,Yanli WANG,Junhu SU.Expression of MTNR1a and MTNR1b genes on HPG axis of male plateau zokor during the breeding and the non?breeding seasons[J].Acta Theriologica Sinica,2022,42(4):410-419. |
| |
| Authors: | Kang AN Mingfang BAO Baohui YAO Yukun KANG Yuchen TAN Yanli WANG Junhu SU |
| |
| Abstract: | The plateau zokor (Eospalax baileyi) is an endemic subterranean rodent species with limited exposure light. Previous study has shown seasonal differences in levels of melatonin secretion in plateau zokors. However, the expression pattern of melatonin receptor genes on hypothalamic?pituitary?gonadal (HPG) axis is not clear. In this study, q–PCR was employed to detect the relative expression levels of genes mRNA of melatonin receptor 1a (MTNR1a)and melatonin receptor 1b (MTNR1b) in hypothalamus, pituitary, and testis of male plateau zokors during the breeding (May) and non?breeding seasons (September). The location of MTNR1a and MTNR1b in testis was determined by immunohistochemical technology, and immunohistochemical positive evaluation was conducted in Image Pro Plus software. The results showed that the relative expression levels of MTNR1a in hypothalamus and pituitary were significantly higher during the breeding season (P < 0.05), while there was no significant difference in the relative expression levels of MTNR1b in different seasons (P > 0.05). The relative expression levels of MTNR1a and MTNR1b in testis during the non?breeding season were higher than that during the breeding season (P < 0.01). The positive signal of MTNR1a in testis was observed in all types of cells except elongated spermatid during the breeding season and in Leydig cells, Sertoli cells, and spermatogonia during the non?breeding season. The positive signal of MTNR1b in testis was observed in all types of cells except spermatogonia and elongated spermatid during the breeding season and in Leydig cells and Sertoli cells during the non?breeding season. The average optical density values of immunopositive signals in MTNR1a and MTNR1b during the non?breeding season were significantly higher compared with the breeding season (P < 0.01). The expression patterns of MTNR1a and MTNR1b on the HPG axis of male plateau zokor during the breeding and the non?breeding seasons revealed the potential role of melatonin in seasonal reproduction regulation. |
| |
| Keywords: | Plateau zokor MTNR1a gene MTNR1b gene Seasonal reproduction |
|
| 点击此处可从《兽类学报》浏览原始摘要信息 |
|
点击此处可从《兽类学报》下载全文 |
