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Diatrack particle tracking software: Review of applications and performance evaluation |
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| Authors: | Pascal Vallotton Antoine M van Oijen Cynthia B Whitchurch Vladimir Gelfand Leslie Yeo Georgios Tsiavaliaris Stephanie Heinrich Elisa Dultz Karsten Weis David Grünwald |
| Affiliation: | 1. Institute of Biochemistry, ETH Zürich, Zürich, Switzerland;2. Faculty of Science Medicine and Health, University of Wollongong, Wollongong, Australia;3. Faculty of Health, University of Technology Sydney, Sydney, Australia;4. Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;5. Civil, Environmental and Chemical Engineering, RMIT University, Melbourne, Australia;6. Medizinische Hochschule Hannover, Hannover, Germany;7. RNA Therapeutics Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts |
| Abstract: | Object tracking is an instrumental tool supporting studies of cellular trafficking. There are three challenges in object tracking: the identification of targets; the precise determination of their position and boundaries; and the assembly of correct trajectories. This last challenge is particularly relevant when dealing with densely populated images with low signal‐to‐noise ratios—conditions that are often encountered in applications such as organelle tracking, virus particle tracking or single‐molecule imaging. We have developed a set of methods that can handle a wide variety of signal complexities. They are compiled into a free software package called Diatrack. Here we review its main features and utility in a range of applications, providing a survey of the dynamic imaging field together with recommendations for effective use. The performance of our framework is shown to compare favorably to a wide selection of custom‐developed algorithms, whether in terms of localization precision, processing speed or correctness of tracks.  |
| Keywords: | biological imaging cell tracking dynamic imaging live cell imaging organelle tracking particle tracking single molecule super‐resolution vesicle tracking |
