DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops |
| |
| Authors: | S Shin S Jung K Heller F Menzel T K Hong J S Shin S H Lee H Lee S Lee |
| |
| Affiliation: | 1. Insect Biosystematics Laboratory, Seoul National University, Seoul, Korea;2. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul, Korea;3. Department of Entomology, North Carolina State University, Raleigh, NC, USA;4. Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Korea;5. Erwin‐Salomon‐Stra?e 25, Quickborn, Germany;6. Senckenberg Deutsches Entomologisches Institut, Müncheberg, Germany;7. Ginseng Resource Research Laboratory, KGC R&D Headquater, Daejeon, Korea;8. Jungbu Regional Office, Animal & Plant Quarantine Agency, Nam‐gu, Incheon, Korea;9. Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea |
| |
| Abstract: | We investigated the usefulness of mitochondrial cytochrome c oxidase (COI) DNA barcoding of the genus Bradysia for the detection of immature stages and cryptic species complex. Although the larvae of some species in this genus are agricultural pests, immature stages are rarely identified due to the lack of key morphological characteristics. We constructed partial sequences of the COI gene for 25 species of Bradysia as a first step towards a DNA barcode. Using these data, Bradysia impatiens, B. procera and B. peraffinis were identified from larval specimens collected, respectively, from paprika, ginseng and oak sawdust beds used for cultivating shiitake. Our findings reveal a complex of three species within the B. tilicola group. These species were all identified as important pest B. ocellaris based on the morphology of male genital structures; however, the interspecific genetic divergence of the COI region was significantly greater (16.1–19.4%) than the intraspecific variation in each species. Therefore, B. ocellaris may consist of at least three species. The results demonstrate that COI DNA barcodes are useful for Bradysia species identification. |
| |
| Keywords: | agricultural pests
Bradysia
COI
cryptic species molecular identification Sciaridae |
|
|
