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周围神经再生过程中靶器官的诱向性作用机制
引用本文:朱道立.周围神经再生过程中靶器官的诱向性作用机制[J].动物学研究,2001,22(6):437-441.
作者姓名:朱道立
作者单位:南通师范学院生命科学与技术系,
摘    要:以细胞培养技术与自然凝胶电泳系统方法证明,在周围神经再生过程中,损伤的坐骨神经远侧端,即起衍生的靶器官诱导神经突起的定向生长。分析探讨与神经诱向性再生相关的活性因子,其结果提示在远侧端神经组织中出现的90kDa蛋白组分具有很强的诱向性作用,诱导神经突起在神经再生过程中能够准确地到达靶器官。由此说明雪旺细胞在神经再生过程中扮演着重要角色。

关 键 词:神经再生  靶器官  诱向性作用机制  坐骨神经  大鼠  周围神经
文章编号:0254-5853(2001)06-0437-05

Neurotropic Effect Mechanism of Target on Peripheral Nerve Regeneration
ZHU Dao,Li.Neurotropic Effect Mechanism of Target on Peripheral Nerve Regeneration[J].Zoological Research,2001,22(6):437-441.
Authors:ZHU Dao  Li
Abstract:The neurotropic effect of the distal stump of transected sciatic nerve on regenerating neurites emerging from the proximal stump was investigated in rats by the co culture of excised dorsal root ganglia and segments of degenerated and nondegenerated sciatic nerves.In all cultures,neurites from the ganglion extended directly towards the degenerated distal stump and not towards the undegenerated nerve segment.Analysis of the supernatants of nerve homogenate by native polyacrylamidegel electrophoresis demonstrated that 235 and 18?kDa proteins were upregulated in the degenerated distal stumps and that 90 and 65?kDa special proteins were expressed by these stumps but not by normal nerve.Placement of the individual protein strips in dissociated cultures of dorsal root ganglion cells showed that the 90?kDa strips possessed strong neurotropic activity.Neurons survived selectively on these strips and outgrowing neurites elongated on the strips paralled to the direction of the band.It is concluded that these bands contain chemotropic substances,the nature of which requires further investigation.
Keywords:Nerve regeneration  Target  Neurotropic effect  Sciatic nerve  Rat  
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