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基于线粒体12S和16S rRNA基因部分序列的角蟾亚科部分属种的系统发育关系
引用本文:江建平,袁富蓉,谢锋,郑中华.基于线粒体12S和16S rRNA基因部分序列的角蟾亚科部分属种的系统发育关系[J].动物学研究,2003,24(4):241-248.
作者姓名:江建平  袁富蓉  谢锋  郑中华
作者单位:1. 中国科学院成都生物研究所,四川,成都,610041
2. 美国德克萨斯大学医学中心生理学系,圣安东尼奥,78229-3900
基金项目:国家自然科学基金资助项目 ( 3 0 0 0 0 0 18),中国科学院生物科学与技术特别支持费资助项目 (STZ -0 1-19),四川省科技 厅重点项目,香港野生动物保护基金会有限公司 (WCF)项目
摘    要:测定了角蟾亚科2属8种(亚种)和外群3种的线粒体12S和16S rRNA基因部分DNA序列,比对后序列长共949bp,其中变异位点数320,简约位点数206。邻接法和最大简约法分析的系统关系树一致表明内群为一单系群,其中腺角蟾首先与其他物种分开;沙坪角蟾与宽头短腿蟾聚为一支;余下的5种(亚种)角蟾组成一支,其中小角蟾短肢亚种的广西种群和香港种群聚为一亚支,另一亚支包括峨眉角蟾、小角蟾指名亚种、尾凸角蟾和重庆武隆的角蟾种,后两种角蟾进化关系最近。本结果支持短肢角蟾为有效种,同时提示腺角蟾、沙坪角蟾与宽头短腿蟾可能隶属3个不同的亚属或属。

关 键 词:角蟾亚科  角蟾属  短腿蟾属  无耳蟾属  rRNA基因  无尾类
文章编号:0254-5853(2003)04-0241-08

Phylogenetic Relationships of Some Species and Genera in Megophryids Inferred from Partial Sequences of Mitochondrial 12S and 16S rRNA Genes
JIANG Jian ping ,YUAN Fu rong ,XIE Feng ,ZHENG Zhong hua.Phylogenetic Relationships of Some Species and Genera in Megophryids Inferred from Partial Sequences of Mitochondrial 12S and 16S rRNA Genes[J].Zoological Research,2003,24(4):241-248.
Authors:JIANG Jian ping    YUAN Fu rong  XIE Feng  ZHENG Zhong hua
Affiliation:JIANG Jian ping 1,3,YUAN Fu rong 2,XIE Feng 1,ZHENG Zhong hua 1
Abstract:Fragments of mitochondrial 12 S and 16 S rRNA genes of 17 individuals were sequenced,including 8 species (subspecies) of 2 genera Megophrys and Brachytarsophrys of Megophrinae,and 3 species used as outgroup including the genera Bombia , Oreolalax and Scutiger .Results pairwised by Clustal W show that there are 949?bp in length with 320 variable sites including 206 parsimony sites.The average contents of T,C,A,and G are 25 6%,24 0%,29 8%,and 20 6%,respectively,and the ratio of transition to transvertion is 1 3.The phylogenetic relationships of these taxa were analyzed using programs of Neighbor Joining and Maximum Parsimony.The results show that the ingroup are clustered together as one clade,of which 3 individuals of M glandulosa were clustered together (BSP=100%) and firstly diverged from the other taxa (BSP=100%),3 individuals of B carinensis were clustered together (BSP=100%) as sister group of M shapingensis ,the rest 5 taxa formed together and included two groups,one consisting of two populations of M minor brachykolos from Longsheng of Guangxi and Hong Kong,the other containing M omeimontis,M minor minor,M caudoprocta and M sp from Wulong of Chongqing,the later two species are closely related.These results support M brachykolos as a valid species rather than a subspecies of M minor ,and suggest that M glandulosa,M shapingensis and B carinensis may belong to three different subgenera or genera.
Keywords:Megophryinae  Megophrys  Brachytarsophrys  Atympanophrys  12  S  rRNA gene  16  S  rRNA gene  Phylogenetic relationship
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