| 中国对虾16 S rRNA基因序列多态性的研究 |
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| 引用本文： | 邱高峰,常林瑞,徐巧婷,方雄英,楼允东.中国对虾16 S rRNA基因序列多态性的研究[J].动物学研究,2000,21(1):35-40. |
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| 作者姓名： | 邱高峰 常林瑞 徐巧婷 方雄英 楼允东 |
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| 作者单位： | 上海水产大学渔业学院,上海 |
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| 基金项目： | 上海市高等学校跨世纪人才培养基金!——“曙光计划”项目，上海市教委高校青年教师学术基金项目 |
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| 摘 要： | 利用ＰＣＲ技术扩增获得中国对虾（Ｐｅｎａｅｕｓ ｃｈｉｎｅｎｓｉｓ）线粒体ＤＮＡ的１６Ｓ ｒＲＮＡ基因片段，通过测定该基因片段的序列，分析了取自我国烟台、长岛近海和宁波养殖的１７只中国对虾遗传多态性。结果发现，不同地理种群存在丰富的ＤＮＡ序列多态性，１７只个体具有１７种基因型，在扩增的长为５２３ｂｐ的基因片段中，共检测到３７个多态性核苷酸位点（７．０７％）。ＵＰＧＭＡ法构建的分子系统树表明不同地理
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| 关 键 词： | 中国对虾 线粒体DNA 16SrRNA 遗传多态性 PCR |
| 文章编号： | ： |
INTRASPECIFIC DNA SEQUENCE POLYMORPHISM IN THE MITOCHONDRIAL 16SrRNA GENE OF THE CHINESE SHRIMP,Penaeus chinensis |
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| QIU Gao-feng.INTRASPECIFIC DNA SEQUENCE POLYMORPHISM IN THE MITOCHONDRIAL 16SrRNA GENE OF THE CHINESE SHRIMP,Penaeus chinensis[J].Zoological Research,2000,21(1):35-40. |
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| Authors: | QIU Gao-feng |
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| Abstract: | The genetic polymorphism in populations of the Chinese shrimp, Penaeus chinensis ,along the coast of China was examined with mitochondrial DNA analysis.A portion of the 16 S ribosomal RNA (16 S ?rRNA) genes from 17 individuals,five from each of samples caught off Changdao,Yantai,Qingdao,and also for two individuals from a cultured stock sampled from Ningbo was amplified with the polymerase chain reaction (PCR) and sequenced.Out of 523 nucleotide sites,37 nucleotide positions (7 07%) were variable and 17 haplotypes were found.Molecular phylogenetic tree constructed by UPGMA method suggested that there were some genetic differential among different geographic populations of Chinese shrimp.Changdao population and Yantai population had a closer genetic relationship.The second was Ningbo population.Qingdao populations diverged to be as one relatively independent branch. |
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| Keywords: | Chinese shrimp Penaeus chinensis Mitochondrial DNA 16 S ?rRNA gene DNA sequence Genetic polymorphism |
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